As an interested layperson who had some exposure to the science of E.coli genetic modification in the early 2000s I'm fascinated by the way the price of analysis has dropped and the speed and accuracy increased. The way you describe the current processes makes me realize just how little I knew about how it was done back then and how much has changed.
I'm curious if the Moderna mass manufacturing method results in similar methylation or is this purely a Pfizer issue.
Anyone else noticed that when you say to a doctor or physician these days that you didn't get the shots, instead of getting a lecture or a tsk-tsk they just get real uncomfortable?
I am amazed at the continuing progress of sequencing technologies, I started with dideoxy Sanger radioactive sequencing with the occasional Maxam-Gilbert for regions where the Klenow struggled... Yes, I am that old. Process 2 DNA is methylated, indeed activating the cGAS-STING pathway, but I am wondering if just this aspect might not have been more beneficial than problematic for the recipients of this soup of nucleic acids. One of the many problems of modified mRNA is the failure to trigger a full antiviral response, by suppressing type I IFN production, and the effect this has on the entire immune system response. From that perspective, activation of the cGAS-STING pathway could be possibly "beneficial" by leading to production of type I IFN, mitigating to some extent the failure of mod mRNA to do so. Not justifying the absolute horror these shots are, of course, just pointing out that this particular defect in their technology is not necessarily problematic.
I think they are relying on it as adjuvant and as Aluminum and other adjuvants are removed, they will replace them with DNA as its an adjuvant you can amplify post application. https://pmc.ncbi.nlm.nih.gov/articles/PMC114371/
Listened to replay of your recent VSRF discussion w Steve Kirsch, (you posted a link above) Backed up to listen again several times to grasp you finding a higher potential for replicating methylation in version2. Is anyone in position of regulating these products even paying attention? Will this “trail of danger” ⚠️ expose ever end? DARPA, DoD and Pharma now so inbred with damn public/private partnerships w govt agencies profiting w royalties, do you see light at end of this dark tunnel?
Sheng-Fowler and Keith Peden study this on Naked DNA.
Lim et al describe the rate of the spontaneous integration once LNPs are involved.
As to your last question, look up chromothripsis. You are welcome to shuffle your genome and be part of the darwian culling that goes on over evolutionary timescales. But again, this post isnt about integration. Its about cGAS-STING. Pharma loves to point the focus on integration.
The switcharoo bugaboo is getting exposed—great work!
As an interested layperson who had some exposure to the science of E.coli genetic modification in the early 2000s I'm fascinated by the way the price of analysis has dropped and the speed and accuracy increased. The way you describe the current processes makes me realize just how little I knew about how it was done back then and how much has changed.
I'm curious if the Moderna mass manufacturing method results in similar methylation or is this purely a Pfizer issue.
Anyone else noticed that when you say to a doctor or physician these days that you didn't get the shots, instead of getting a lecture or a tsk-tsk they just get real uncomfortable?
Sloppy science or purposeful? Me thinks 🧐, purposeful 🤬
I am amazed at the continuing progress of sequencing technologies, I started with dideoxy Sanger radioactive sequencing with the occasional Maxam-Gilbert for regions where the Klenow struggled... Yes, I am that old. Process 2 DNA is methylated, indeed activating the cGAS-STING pathway, but I am wondering if just this aspect might not have been more beneficial than problematic for the recipients of this soup of nucleic acids. One of the many problems of modified mRNA is the failure to trigger a full antiviral response, by suppressing type I IFN production, and the effect this has on the entire immune system response. From that perspective, activation of the cGAS-STING pathway could be possibly "beneficial" by leading to production of type I IFN, mitigating to some extent the failure of mod mRNA to do so. Not justifying the absolute horror these shots are, of course, just pointing out that this particular defect in their technology is not necessarily problematic.
I think they are relying on it as adjuvant and as Aluminum and other adjuvants are removed, they will replace them with DNA as its an adjuvant you can amplify post application. https://pmc.ncbi.nlm.nih.gov/articles/PMC114371/
Brought this to mind...
https://www.annualreviews.org/content/journals/10.1146/annurev-genom-010323-011239
Pfizer sells it much cheaper in pocket size: https://open.substack.com/pub/controlstudies/p/minion?utm_source=share&utm_medium=android&r=31oy60
No surprise Jamie takes it on...
Listened to replay of your recent VSRF discussion w Steve Kirsch, (you posted a link above) Backed up to listen again several times to grasp you finding a higher potential for replicating methylation in version2. Is anyone in position of regulating these products even paying attention? Will this “trail of danger” ⚠️ expose ever end? DARPA, DoD and Pharma now so inbred with damn public/private partnerships w govt agencies profiting w royalties, do you see light at end of this dark tunnel?
Our papers spell this out.
Sheng-Fowler and Keith Peden study this on Naked DNA.
Lim et al describe the rate of the spontaneous integration once LNPs are involved.
As to your last question, look up chromothripsis. You are welcome to shuffle your genome and be part of the darwian culling that goes on over evolutionary timescales. But again, this post isnt about integration. Its about cGAS-STING. Pharma loves to point the focus on integration.
Sheng-Fowler
https://pubmed.ncbi.nlm.nih.gov/19285882/