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Amazing that Moderna is openly admitting things like that p53 suppression is a possibility- but this didn’t alarm anyone at the DoD or government before mandating it across many sectors? I had cancer 1.5 years ago and it is in my chart that i won’t take an mrna jab because it specifically could be a p53 suppressor. At first they looked at me like i wore tinfoil on my head, these days they are more subdued. I keep hoping they will openly discuss it. i even told a med student doing rotation to look you up and this particular point. It’s a well known university hospital so i have to name drop because these kids are only into credentials and i clearly don’t have them 😂

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thank you Kevin,

for us still questioning cats: https://i.postimg.cc/z3mjT3VY/shutterstock-2009131298.jpg

.. does this mean, intentional design?

and .. are we closer to knowing whether this ORF is translating/producing silk-like proteins in humans

and .. have we any ideas for how we can test populations for (a) still having this sequence someplace inside them and (b), whether it is (or was) translating this Spidroin-like protein

as one of the few lawyers in the room i am trying to keep up to speed with your science in terms i can relate to colleagues and others involved in proceedings afoot (which you know about), and others we are preparing

merci beacoup

Julian

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am i missing something?

leucine codons UUA 7.7 and CUA 7.2 are the less used codon in humans, so they don't use it.

serine UCG 4.4 ACU 12.1 and UCA 12.2 are the 3 less frequent , also not used.

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Barring some rationale for exclusively using those codons for Leu and Ser, hard to accept it as chance. Spike is a very long gene for in vitro transcription, so in generating the mRNA transcripts, I can see the possible complications from hairpins and unpredicted duplexes with the template. Perhaps part of their production optimization involved using in vitro translation to simply look for a protein product of the approximate size of spike (using sds-page). If your transcript batch (all of ~equal length) is 100% sense strand spike, you get mostly the correct size protein produced. If the mRNA batch is 75% sense spike, 25% chimera, you would expect 25% of the protein produced as shorter fragments (IF they used typical Leu and Ser codons optimized for human expression). Let's say they observed this and suspected a translation efficiency problem (e.g. mRNA secondary structure) that could be addressed by codon usage. Someone clever in the group takes this on but has a better notion for the problem: focusing on the Leu and Ser codons.........viola almost all protein synthesized with the expected size ORF.

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