Deep sequencing of the Moderna and Pfizer bivalent vaccines identifies contamination of expression vectors designed for plasmid amplification in bacteria.
Hi thank you for your work, I took the Pfizer bivalent. I’m not fully understanding but if you could tell me if this means if I get a bacterial infection my body will resist antibiotics ?
I would recommend you stop taking these truly worthless and very harmful injections. The harms are so many, they are getting difficult to quantify. Just take early treatment, keep your serum vitamin D levels about 60-80 ng/ml and eat a life-giving, healthy organic diet, and walk in the sun when you can.
Yes unfortunately here we have to take 3 shots as nurses. I tried to skip the booster but was eventually told to get it the bivalent but definitely no more and thank you for your advice.
I have some understanding of the pressure you are under. I also understand that medical students are indoctrinated (no pun intended) to believe that vaccines are miraculous and never to be questioned. Perhaps read "Dissolving Illusions" by Dr. Suzanne Humphries (quite a tome but worth it) and/or "Neither Safe nor Effective" by Dr. Colleen Huber to begin your deprogramming. There are several posts by different authors on Substack about clearing the body of the toxins in these shots. Nattokinase is mentioned in a few and might be a good place to start.
Northfield Hospital in Minnesota was successfully sued by fired employees over their mandate, so that may serve as a template for future legal action. Take care and God bless.
I’m so sorry, David. If you ever get to join a class action suit, I’d myself would be sorely tempted to do it. May your health be good going forward for you, your family, and friends.
You don’t have to take anything. You can’t comply your way out of tyranny. There will be more mandates. Say no and walk away. Other spineless nurses need to do the same. They would have to drop the “mandate “ if enough people just said no.
One of the most important pieces of independent research carried out since the pandemic began. Why it’s being left to independent researcher’s and not the manufacturer’s or regulators to make this information publicly available is an open question for our times. Thank you Kevin for this work, every single person needs to be aware.
Well done. I strongly recommend submission for peer review to timestamp these important results.
I am happy to consider a paper for peer review in Science, Public Health Policy & the Law. The proteins of the replication-competent can also now be considered potential triggers of pathogenic priming by cross-reactive autoreactogenic epitopes. - James Lyons-Weiler
The methods used sequenced both RNA and DNA present in the vial.
The DNA has Watson and Crick strands present and the mRNA is exclusively crick strand so it shows up like a soar thumb..
SV40 is one of the most common promoters used in mammalian expression systems. I’m just not well versed on how many times it’s been introduced via transformation of billions of people.
With the mRNA, did you find anything in it (proteins being coded for) that were not supposed to be there? This has been a continual topic of debate (if a bad protein was there). My impression from reading this is that you did not, but I want to try and make sure
Additionally, did you find signs the mRNA sequence in the vaccines was slightly erroneous from the spike protein it was supposed to code for?
In regards to the SV40 promoter, are there any known consequences of introducing it to humans (as the entire virus was speculatively ascribed to being able to cause cancer)?
That is a more complicated question that we are still digging into. The error rate of T7 polymerase when incorporating N1-methyl Pseudo U is known to increase 200-300%. This would imply an error every 3000-4000 bases. This implies a mistake in every molecule made. These errors are then fed into ribosomes which make even more mistakes than T7 polymerase trying to read N1 methyl Pseudo U. Bruce pattersons lab has found mutated spike in PASC patients but not in C19 patients. I think those are a result of these mutations
I’m still looking at the fidelity question but hoping a few more people can now beat me to answer with the data public. It’s helpful to have a few more eyes make an attempt at the more complicated analysis.
The vector just has the SV40 promoter not the whole SV40 genome so I don’t think we pin the cancer issue on it.
I don’t have a mass spec to address the peptide sequencing questions.
I would be very wary of taking government (Australian) figures seriously. 96% vaccinated is ludicrously exaggerated. Anecdotal but Catherine Austin Fitts makes the same point: the middle class are, and the working class aren't.
Fitts and I going off observation that of tradesmen coming to the house almost none are vaxed, indeed have very firm views on the subject, while the entirety of our middle-class families are. Online this demographic seems more or less to hold.
I assume that govt/msm inflation of figures is tactical, to make the unvaxed feel isolated. Someone using Govt figures for Australia came up with only 26% fully vaxxed. Would pass that on except it may be buried under more recent posts.
Thanks for a great article by the way. Should have started off with that !
err.....isn't this the sort of basic QA stuff that should be done BEFORE starting a Phase I clinical trial??
Shouldn't rigorous analysis of this sort of QA be the primary function of regulators when deciding whether to issue a license to perform early stage clinical trials??
Why is this being done after a billion and more people have been injected with the stuff??
'Let's all expose the world to a nuclear bomb and then learn afterwards what the effects of that sort of radiation exposure has on the human immune system....'
It’s ironic you state the nuclear issue. That is exactly what they did and will continue to do. They irradiated us, sprayed us from above. Then lie. The documents will remain classified until the last general or mad scientist is long dead. No tribunal no accountability. I remember Iraqi soldiers being accused of throwing the babies from the incubators. All a lie. It was broadcast all over the US to justify the first invasion. I also remember a couple of skyscrapers falling like a pancake from jet fuel. Not a controlled demolition with thermite explosives placed throughout the structures supporting beams. Then building six just pancakes. How strange. Then all the engineers who spoke out against it mysteriously died. Yellow cake. WMD’s The gulf of Tolkien incident. Operation Northwoods. I can go on and on. If it is on the news it is propaganda. Disregard it. If they claim aliens are landing it’s BS. Until we Learn from the past we are doomed to relive it over and over. The problem is people are incapable of critical thinking. And do not truly ask questions. They simply regurgitate talking points they hear from television. Shall I quote William Randolph Hearst? “Provide me the photographs I will provide the war” The government will ultimately shut down the independent labs who do real research. And force the state approved results. You will lose your permits and your licenses. While ultimately being disgraced. No due process no right to trial by jury. And if you are allowed a trial it will be a state sanctioned kangaroo court with a state picked jury. I suggest you start watching the twilight zone and or the outer limits for a glimpse into the future. 1984 is a good read. So is a brave new world. Then read the Bible. Very good reading material.
Wow - the more you look the worse it gets! Thanks for doing this important work and for setting an example. It's time for the worlds’ scientists to get back to work as in applying the tools available to inquire into these areas. It IS possible to know much of this - you just have to look and as you've pointed out it doesn't require a lot of money. Tens of thousands (100s?) of labs have everything they need. I have a background in molecular biology (PhD 1995) and I share Jessica's enthusiasm for how cool this stuff is (I contributed to identifying gene mutations responsible for heritable diseases in mice, humans and dogs and it's pretty exciting work). And so I'm familiar with many of the techniques that you've discussed in this post but doing things in the lab is one thing and actually injecting people with foreign genetically manipulated material another. You've brought up some additional questions that need answers and the effects on the microbiome is a big one if this were to become a reservoir for endless spike production as well as the antibiotic resistance potential as bacteria regularly share genetic material. As we get more and more pieces to the puzzle of this horror, I have to mention the link Jessica recently posted to a roundtable with Michael Yeadon called Toxic by Design. So many bad ideas THAT WERE KNOWN combined in these shots (as well as a few unknown – just in case). Oh, the humanity!
I am especially concerned about the possible microbiome effects. I was bamboozled into getting the original Moderna shots, and about a year later my gut bugs started misbehaving, after a decade of trouble-free performance helped along by a variety of fermented foods.
So strange that all of the "accidents" like all of the spike-protein mutations seem to "coincidentally" have specific functionalities that are harmful, not just random sludge...
Multiple antibiotic resistance genes! Will wonders never cease?
I'll include this in my next blog post, likely tomorrow.
God help us against this witch’s brew of Lord knows what? To take an injection from these vials is like playing Russian Roulette with a psychopathic demon’s gun. I feel so sorry for the vast number of my family, friends, coworkers and fellow citizens here in Canada who fell for this ‘kill-shot’. It’s heart breaking and it cannot be undone. How many trusting children will suffer for their parents manipulated fear? The local city’s newspaper, radio and television stations broadcast death counts every day in ceaseless propaganda for over a year. 😢 I don’t recognize my country anymore. What is yet to come? I’m beginning to fear the ‘Zombie apocalypse’ hyped for years in the media is a foreshadowing of what is to come as the genetic results of this misbegotten poison jab accumulates to critical mass. Sorry to be such a downer but this article is alarming in its potential scope.
Are there any straightforward reference articles or substacks explaining this kind of thing for the layman? I see the graph and can well believe that it shows something seriously off, but have no idea how to interpret or contextualise the data I'm seeing there. Thanks!
Katherine Watt does thorough legal aspects, Jessica Rose has excellent diagrams and explanations, Sasha Latypova explains a bit of both. All on substack. You could also read Steve Kirsch's articles. I know a lot of these articles look like a foreign language to laypersons. I think that is part of the way the powers-that-shouldn't-be try to 'control' humanity. Bless you for wanting to fully understand what is happening to people.
George Gammon, Greg Mannarino, and Steven Van Metre are additional resources I check out regularly.
FDIC QBP Q42022 came out today. The FDIC also COMPLETELY changed the format and removed graphs they normally have. (Sneaky little bastards. They know too many people are watching.)
You'll be happy to know the banks ONLY lost 10% less than Q32022 on "Unrealized Losses on Securities." $62 billion as opposed to $69.5 billion. LOL FHLB Advances are 211% higher than Q42021.
Titus correctly correlated FHLB Advances increases with panic borrowing by the banks. Not to mention the Fed is increasing rates while decreasing its balance sheet. Unprecedented. Not looking good...
They lost their asses in the stonk market to the tune of about $1.3 trillion in 2022, loan delinquencies are going up, and customers aren't borrowing as much because of higher rates.
A "bail in" is when a financial institution seizes customer accounts to make themselves whole for the "greater good." (See Greece in 2009, Cyprus in 2012, and recent bank failures. More bank failures are coming by the way because the Federal Reserve is actively at war with banks that are not "systemically important to them.)
You are essentially an unsecured depositor in a bank's eyes. In the past, banks had to keep 10% of total deposits on reserve in cash. The other 90% is lent out elsewhere. In March 2020, that reserve requirement was revised to 0% meaning banks could technically have ZERO cash on hand. See what happens when you make a large withdrawal especially early in the week.
It gets better. The FDIC is underfunded by 98%. That "up to $250K is insured" is a complete lie.
It takes a little bit more work, but be out of the system as much as you can by keeping minimal balances and depositing as needed. The rug pull is coming.
Thanks...and where exactly should we take our cash? Crypto isn't looking too stable either! Paper won't be worth much and there is only so much gold or silver you can buy and store (where? in a safe in your home?) Credit unions are better but by how much?!
PHYSICAL gold and silver along with some lead and brass...
Silver has more "upside" since the GSR is currently off by a factor of 6 based on historical numbers, but gold takes up less space.
Remember to have it in YOUR CONTROL. Safe deposit boxes will be inaccessible too when they decide to have a "bank holiday." Don't fall for that scam on TV where your precious metals "are stored in our secure vaults." If you don't HOLD it, you don't OWN it. Become your own central bank.
I would include 2019 as well because they were already promoting propaganda from China about this mystery virus requiring quarantines … recall some articles from Daily Mail
Most begin counting years at 21 and skip over the first year thinking that this is correct because of the 3 at the end of 2023 so they mistakenly conclude three years in. Just as most don't count their birth year and begin counting bdays at the beginning of the second year of life. So, however old you are, add a year!
Due to the fact that the Moderna as well as the Pfizer plasmid carry a f1 ori, have you tried to use the vacine, maybe upon opening of the LNPs, for direct transformation of E. coli competent cells?
This would be a more direct proof for intact plasmids that can mediate resistance. In addition you would obtain single plasmids upon replication in E coli cells for resequencing and resolving the problems with the different spike protein variants.
We just prepped with a method we have here to sequence the genomes of colonies.
The plasmid come for free with Illumina sequencing.
I’m a bit skeptical the T7 promoter will leak much RNA in this cell line but in a week or two we’ll have RT-qPCR primers and probes that target the vector and insert to answer those questions.
You took quite a risk doing this work, tampering with government property. On behalf of humanity, thank you.
There was nothing written on the vials that declared it to be government property.
If we can’t sequence what they want injected into our kids, there is no freedom in this world.
Hi thank you for your work, I took the Pfizer bivalent. I’m not fully understanding but if you could tell me if this means if I get a bacterial infection my body will resist antibiotics ?
I would recommend you stop taking these truly worthless and very harmful injections. The harms are so many, they are getting difficult to quantify. Just take early treatment, keep your serum vitamin D levels about 60-80 ng/ml and eat a life-giving, healthy organic diet, and walk in the sun when you can.
Yes unfortunately here we have to take 3 shots as nurses. I tried to skip the booster but was eventually told to get it the bivalent but definitely no more and thank you for your advice.
I have some understanding of the pressure you are under. I also understand that medical students are indoctrinated (no pun intended) to believe that vaccines are miraculous and never to be questioned. Perhaps read "Dissolving Illusions" by Dr. Suzanne Humphries (quite a tome but worth it) and/or "Neither Safe nor Effective" by Dr. Colleen Huber to begin your deprogramming. There are several posts by different authors on Substack about clearing the body of the toxins in these shots. Nattokinase is mentioned in a few and might be a good place to start.
Northfield Hospital in Minnesota was successfully sued by fired employees over their mandate, so that may serve as a template for future legal action. Take care and God bless.
I’m so sorry, David. If you ever get to join a class action suit, I’d myself would be sorely tempted to do it. May your health be good going forward for you, your family, and friends.
You don’t have to take anything. You can’t comply your way out of tyranny. There will be more mandates. Say no and walk away. Other spineless nurses need to do the same. They would have to drop the “mandate “ if enough people just said no.
Gonna Substack this Substack. Man oh man.
One of the most important pieces of independent research carried out since the pandemic began. Why it’s being left to independent researcher’s and not the manufacturer’s or regulators to make this information publicly available is an open question for our times. Thank you Kevin for this work, every single person needs to be aware.
It's because it is a DOD operation and these companies are the fall guys! Bait and switch!
Well done. I strongly recommend submission for peer review to timestamp these important results.
I am happy to consider a paper for peer review in Science, Public Health Policy & the Law. The proteins of the replication-competent can also now be considered potential triggers of pathogenic priming by cross-reactive autoreactogenic epitopes. - James Lyons-Weiler
Hi this is great. If it's ok with you I'd like to include some of this in my article.
As a few questions I feel I should know the answer to:
•Have they been able to demonstrate any types of issues exist with introducing the SV40 promoter into humans?
•Did you also sequence the mRNA present (I think you did, but I just want to make sure).
The methods used sequenced both RNA and DNA present in the vial.
The DNA has Watson and Crick strands present and the mRNA is exclusively crick strand so it shows up like a soar thumb..
SV40 is one of the most common promoters used in mammalian expression systems. I’m just not well versed on how many times it’s been introduced via transformation of billions of people.
The central questions I had from this were:
With the mRNA, did you find anything in it (proteins being coded for) that were not supposed to be there? This has been a continual topic of debate (if a bad protein was there). My impression from reading this is that you did not, but I want to try and make sure
Additionally, did you find signs the mRNA sequence in the vaccines was slightly erroneous from the spike protein it was supposed to code for?
In regards to the SV40 promoter, are there any known consequences of introducing it to humans (as the entire virus was speculatively ascribed to being able to cause cancer)?
That is a more complicated question that we are still digging into. The error rate of T7 polymerase when incorporating N1-methyl Pseudo U is known to increase 200-300%. This would imply an error every 3000-4000 bases. This implies a mistake in every molecule made. These errors are then fed into ribosomes which make even more mistakes than T7 polymerase trying to read N1 methyl Pseudo U. Bruce pattersons lab has found mutated spike in PASC patients but not in C19 patients. I think those are a result of these mutations
Got it! Really appreciate it. Any thoughts on the other two questions?
I’m still looking at the fidelity question but hoping a few more people can now beat me to answer with the data public. It’s helpful to have a few more eyes make an attempt at the more complicated analysis.
The vector just has the SV40 promoter not the whole SV40 genome so I don’t think we pin the cancer issue on it.
I don’t have a mass spec to address the peptide sequencing questions.
Enjoyed this discussion. Thanks
And yet, we are the heroes we are looking for. Welcome to the Thunderdome all.
I would be very wary of taking government (Australian) figures seriously. 96% vaccinated is ludicrously exaggerated. Anecdotal but Catherine Austin Fitts makes the same point: the middle class are, and the working class aren't.
Good to know. I’m merely going off of dashboards and other Substack threads on those numbers.
Fitts and I going off observation that of tradesmen coming to the house almost none are vaxed, indeed have very firm views on the subject, while the entirety of our middle-class families are. Online this demographic seems more or less to hold.
I assume that govt/msm inflation of figures is tactical, to make the unvaxed feel isolated. Someone using Govt figures for Australia came up with only 26% fully vaxxed. Would pass that on except it may be buried under more recent posts.
Thanks for a great article by the way. Should have started off with that !
That's really fantastic news. It's funny how even such a basic number can't be trusted.
I'm also confused by which countries are reporting total population Vax numbers and which are reporting adult Vax numbers while ignoring children.
That's what I said. See above comment.
I agree. Cannot trust their figures. They want us to believe we are the few!
err.....isn't this the sort of basic QA stuff that should be done BEFORE starting a Phase I clinical trial??
Shouldn't rigorous analysis of this sort of QA be the primary function of regulators when deciding whether to issue a license to perform early stage clinical trials??
Why is this being done after a billion and more people have been injected with the stuff??
'Let's all expose the world to a nuclear bomb and then learn afterwards what the effects of that sort of radiation exposure has on the human immune system....'
It’s ironic you state the nuclear issue. That is exactly what they did and will continue to do. They irradiated us, sprayed us from above. Then lie. The documents will remain classified until the last general or mad scientist is long dead. No tribunal no accountability. I remember Iraqi soldiers being accused of throwing the babies from the incubators. All a lie. It was broadcast all over the US to justify the first invasion. I also remember a couple of skyscrapers falling like a pancake from jet fuel. Not a controlled demolition with thermite explosives placed throughout the structures supporting beams. Then building six just pancakes. How strange. Then all the engineers who spoke out against it mysteriously died. Yellow cake. WMD’s The gulf of Tolkien incident. Operation Northwoods. I can go on and on. If it is on the news it is propaganda. Disregard it. If they claim aliens are landing it’s BS. Until we Learn from the past we are doomed to relive it over and over. The problem is people are incapable of critical thinking. And do not truly ask questions. They simply regurgitate talking points they hear from television. Shall I quote William Randolph Hearst? “Provide me the photographs I will provide the war” The government will ultimately shut down the independent labs who do real research. And force the state approved results. You will lose your permits and your licenses. While ultimately being disgraced. No due process no right to trial by jury. And if you are allowed a trial it will be a state sanctioned kangaroo court with a state picked jury. I suggest you start watching the twilight zone and or the outer limits for a glimpse into the future. 1984 is a good read. So is a brave new world. Then read the Bible. Very good reading material.
https://twitter.com/pandata19/status/1633458251403763713?s=46&t=Tsl-3F33wzmMqLy5S3XYVg
Wow - the more you look the worse it gets! Thanks for doing this important work and for setting an example. It's time for the worlds’ scientists to get back to work as in applying the tools available to inquire into these areas. It IS possible to know much of this - you just have to look and as you've pointed out it doesn't require a lot of money. Tens of thousands (100s?) of labs have everything they need. I have a background in molecular biology (PhD 1995) and I share Jessica's enthusiasm for how cool this stuff is (I contributed to identifying gene mutations responsible for heritable diseases in mice, humans and dogs and it's pretty exciting work). And so I'm familiar with many of the techniques that you've discussed in this post but doing things in the lab is one thing and actually injecting people with foreign genetically manipulated material another. You've brought up some additional questions that need answers and the effects on the microbiome is a big one if this were to become a reservoir for endless spike production as well as the antibiotic resistance potential as bacteria regularly share genetic material. As we get more and more pieces to the puzzle of this horror, I have to mention the link Jessica recently posted to a roundtable with Michael Yeadon called Toxic by Design. So many bad ideas THAT WERE KNOWN combined in these shots (as well as a few unknown – just in case). Oh, the humanity!
I am especially concerned about the possible microbiome effects. I was bamboozled into getting the original Moderna shots, and about a year later my gut bugs started misbehaving, after a decade of trouble-free performance helped along by a variety of fermented foods.
So strange that all of the "accidents" like all of the spike-protein mutations seem to "coincidentally" have specific functionalities that are harmful, not just random sludge...
Multiple antibiotic resistance genes! Will wonders never cease?
I'll include this in my next blog post, likely tomorrow.
There is no Spike. PsyOp
Lol
God help us against this witch’s brew of Lord knows what? To take an injection from these vials is like playing Russian Roulette with a psychopathic demon’s gun. I feel so sorry for the vast number of my family, friends, coworkers and fellow citizens here in Canada who fell for this ‘kill-shot’. It’s heart breaking and it cannot be undone. How many trusting children will suffer for their parents manipulated fear? The local city’s newspaper, radio and television stations broadcast death counts every day in ceaseless propaganda for over a year. 😢 I don’t recognize my country anymore. What is yet to come? I’m beginning to fear the ‘Zombie apocalypse’ hyped for years in the media is a foreshadowing of what is to come as the genetic results of this misbegotten poison jab accumulates to critical mass. Sorry to be such a downer but this article is alarming in its potential scope.
You are not alone in suffering from this living nightmare
Just when I was hoping to have a good night’s sleep!
The jabs were always the intended illness vectors.
The picture below is the REASON for the last 3+ years of insanity.
https://files.catbox.moe/lgumsu.PNG
The banks are BROKE.
Are there any straightforward reference articles or substacks explaining this kind of thing for the layman? I see the graph and can well believe that it shows something seriously off, but have no idea how to interpret or contextualise the data I'm seeing there. Thanks!
Katherine Watt does thorough legal aspects, Jessica Rose has excellent diagrams and explanations, Sasha Latypova explains a bit of both. All on substack. You could also read Steve Kirsch's articles. I know a lot of these articles look like a foreign language to laypersons. I think that is part of the way the powers-that-shouldn't-be try to 'control' humanity. Bless you for wanting to fully understand what is happening to people.
John Titus aka Best Evidence
https://www.youtube.com/@BestEvidence/videos
George Gammon, Greg Mannarino, and Steven Van Metre are additional resources I check out regularly.
FDIC QBP Q42022 came out today. The FDIC also COMPLETELY changed the format and removed graphs they normally have. (Sneaky little bastards. They know too many people are watching.)
You'll be happy to know the banks ONLY lost 10% less than Q32022 on "Unrealized Losses on Securities." $62 billion as opposed to $69.5 billion. LOL FHLB Advances are 211% higher than Q42021.
https://www.fdic.gov/analysis/quarterly-banking-profile/qbp/2022dec/qbp.pdf#page=1
Titus correctly correlated FHLB Advances increases with panic borrowing by the banks. Not to mention the Fed is increasing rates while decreasing its balance sheet. Unprecedented. Not looking good...
Scary graph. Thank you. One has to wonder, how can the banks be broke when the FED just prints out more $$?
They lost their asses in the stonk market to the tune of about $1.3 trillion in 2022, loan delinquencies are going up, and customers aren't borrowing as much because of higher rates.
https://files.catbox.moe/2n6lt2.PNG
https://www.fdic.gov/analysis/quarterly-banking-profile/qbp/2022sep/qbp.pdf#page=1
As of right now, the Fed isn't stepping in because QT has actually been going on since Spring 2022. (Dark blue M2 supply line in my last post.)
Get your cash out of the banks because the "solution" this time around will involve BAIL INS.
BAIL INS? What is that?
A "bail in" is when a financial institution seizes customer accounts to make themselves whole for the "greater good." (See Greece in 2009, Cyprus in 2012, and recent bank failures. More bank failures are coming by the way because the Federal Reserve is actively at war with banks that are not "systemically important to them.)
You are essentially an unsecured depositor in a bank's eyes. In the past, banks had to keep 10% of total deposits on reserve in cash. The other 90% is lent out elsewhere. In March 2020, that reserve requirement was revised to 0% meaning banks could technically have ZERO cash on hand. See what happens when you make a large withdrawal especially early in the week.
It gets better. The FDIC is underfunded by 98%. That "up to $250K is insured" is a complete lie.
It takes a little bit more work, but be out of the system as much as you can by keeping minimal balances and depositing as needed. The rug pull is coming.
Thanks...and where exactly should we take our cash? Crypto isn't looking too stable either! Paper won't be worth much and there is only so much gold or silver you can buy and store (where? in a safe in your home?) Credit unions are better but by how much?!
PHYSICAL gold and silver along with some lead and brass...
Silver has more "upside" since the GSR is currently off by a factor of 6 based on historical numbers, but gold takes up less space.
Remember to have it in YOUR CONTROL. Safe deposit boxes will be inaccessible too when they decide to have a "bank holiday." Don't fall for that scam on TV where your precious metals "are stored in our secure vaults." If you don't HOLD it, you don't OWN it. Become your own central bank.
This can not be, They are only waiting for the due date of their all-in call option on a CCS plant at the mines in Soledar.
We are actually in the 4th year of this madness.
Year 1 - 2020
Year 2 - 2021
Year 3 - 2022
Year 4 - 2023
I would include 2019 as well because they were already promoting propaganda from China about this mystery virus requiring quarantines … recall some articles from Daily Mail
Especially if it actually was created sooner!
Most begin counting years at 21 and skip over the first year thinking that this is correct because of the 3 at the end of 2023 so they mistakenly conclude three years in. Just as most don't count their birth year and begin counting bdays at the beginning of the second year of life. So, however old you are, add a year!
Thank you for the approach and for sharing.
Due to the fact that the Moderna as well as the Pfizer plasmid carry a f1 ori, have you tried to use the vacine, maybe upon opening of the LNPs, for direct transformation of E. coli competent cells?
This would be a more direct proof for intact plasmids that can mediate resistance. In addition you would obtain single plasmids upon replication in E coli cells for resequencing and resolving the problems with the different spike protein variants.
Good advice.
They do transform NEBexpress Kan sensitive ecoli cells and we have these colonies made into sequencing libraries.
They gDNA preps from these colonies have plasmids. Just need sequence confirmation that it’s not some artifact.
Thank you for letting me know! If you have done the propper controls, growth of colonies of E. coli on Kan tells you a lot.
Why do you go for gDNA and not a simple plasmid prep? The f1 ori should result in high copy number and you get rid off the gDNA for sequencing.
Even if you would find modified plasmids (smaller in size, different in sequence) the argument of transmission of a resistance gene still holds true.
Moreover, nobody knows what else can be transcript in the microbiome.
Anyhow, very interesting and thanks for digging deeper.
We just prepped with a method we have here to sequence the genomes of colonies.
The plasmid come for free with Illumina sequencing.
I’m a bit skeptical the T7 promoter will leak much RNA in this cell line but in a week or two we’ll have RT-qPCR primers and probes that target the vector and insert to answer those questions.