Nuclear permeability during cell division

Flattening the "won't get to the nucleus" canard

In late May I had the honor of speaking with Sucharit Bhakdi, MD regarding dsDNA contamination in the vaccines.

First, we need to do some numbers.

The 100ug Moderna shots deliver over 40 trillion mRNA molecules (14T for Pfizer) and the number of LNPs per shot is reported to be 10-50 billion. For easy math, lets assume 40 billion LNPs. This is roughly 1,000 mRNAs in each LNP.

There over 800 N1-methyl-PseudoUs (m1Ψ) in each mRNA of Pfizer and likely an equal number in Moderna mRNAs for C19 vaccination. 800,000 m1Ψ per cell. This may influence the PUS (Pseudo Uridine Synthase) pathway. This pathway is very thinly published on. This is a very nascent field in the Epigenetics of RNAs. While we understand which enzyme modify Uracil to Ψ to m1Ψ , less is known about the processes that reverse this.

Most other RNA methylation systems (methyl A, and methyl C) have RWE pathways (Read, Write, Erase) understood. There are proteins identified that recognize the modified base (read), ones that make the modifications (write), and proteins identified that erase the methylation. The reading and writing for Ψ and m1Ψ have been identified but the erasing is still a mystery.

In addition to these transfected vaccine mRNAs, we now know there is substantial amount of dsDNA coming along for the ride. It is packaged.

If dsDNA contamination is 1%-10% of these mRNA numbers we are injecting 10-100 dsDNA molecules into each cell transfected. Likely 40 billion cells if 1 LNP infects a single cell. In reality this number is likely much higher at the site of the injection and diffuses to lower MOI (multiplicity of infection) as it distributes to nearly every tissue in the body. This is 0.13% of the 30 trillion cells in your body.

Cells are being transfected with 10-100 dsDNA molecules each. These DNAs are likely fragmented and short dsDNA given what we know with the low Adverse event batches analyzed to date. They may be longer and more intact in the poor batches analyzed by Schmeling et al.

Will the dsDNA contaminants ever make it to the nuclease? About 1/40th of them will contain an SV40 promoter with a nuclear localization signal. The dsDNA isn’t all SV40 DNA. It’s 7,810bp of sequence for Pfizer. If the dsDNA is ~200bp in size The SV40 region is just ~1/40th of the dsDNA in the contamination.

But Dr. Bhakdi pointed out something I hadn’t considered. During cell division the nucleus disassembles exposing the nuclear genome to the cytoplasm and transcription is still active during this time window. Most genome integrations occur in regions being actively transcribed. This would imply all fragments of the 7,810bp plasmid DNA is on the table for integration.

The video going over this has been viewed nearly half a million times.

While this data may be damning for the current formulations of the vaccines, we must keep in mind that this is in no way a check mate. This is a very fixable problem. Other nucleases exist that are likely not as sensitive to RNA/DNA hybrid inhibition seen with DNase I. Pharma will clean this up or change the regs (which ever is cheaper).

Same is true with the Spike protein. They are already publishing papers swapping spike for nucleocapsid (also has amyloidagenic sequences). It’s very likely that they will perform this swap before any humility or reflection is taken on the delivery platform.

Transfecting this many epithelial cells with any peptide that labels it for destruction may produce many of the adverse events described. The trials have no LNP vehicle controls. Give

Marc Girardot

substack a read. The IM injection route of this platform is likely damned for achieving mucosal immunity and comes with significant risks of transfecting and napalming your vasculature or what ever target organ it distributes to.

And make no mistakes about this being a One and Done technology. It is a platform they plan to reprogram for the veterinary market and most other human pathogens including cancer.

Dr Karina Acevedo-Whitehouse gave an interesting presentation at the Better Ways conference that went over these risks. A few select slides of hers below.

We know the Ψ provides mRNA stability. We also know there is stable dsDNA present.

The biodistribution is diverse but may vary tremendously patient to patient based on the route of the circulatory system hit by non-aspirating vaccination.

What is triggered intracellularly is bit of mystery given the modification made and the contamination present.

Many of the detractors to this work will Namaste over the dsDNA being too little to matter. Remind them of using qPCR to detect C19 sgRNA from your mucosa. They were calling some positive for CTs under 40.

Violating civil rights and wrecking the economy came with far lower standards than the contaminants of their pharmaceutical partners. The dsDNA contaminant in these vaccines is a CT of 20 for 1/300th of the vaccine dose. Thats over a millions times higher (300 million for a CT of 40) amounts of contaminating nucleic acid than what would trigger a CT 40 sgRNA qPCR test.

With qPCR, we finally have a quantitative Political Corruption Readout as we can measure precisely the size of their hollowed out house of hypocrisy.

The contaminant is injected and thus evades your mucosal defenses. This is much different than surveying sgRNA on the outside of your mucosal defenses as seen with nasal swabs in C19 RT-qPCR.

qPCR = quantitative Political Corruption Readout

A few excerpts from Robert Weinberg’s text book on Ocogenesis were sent to me from some colleagues in Japan. This is a reminder of the carcinogenic risks of genome integration which is far harder for your body to clean up when these injections also lower your white blood cell counts.

In conclusion,

The 330ng/mg limit at the EMA deserves more scrutiny. What was the reasoning behind this limit and did it consider the dsDNA would be packaged in transfection ready LNPs?