Introduction Using methods described earlier, we sequenced the Pfizer monovalent BNT162b2 vaccines and assembled these reads with Megahit. A 7,754 base pair spike encoded plasmid was assembled with a truncated Poly A tract and a previously described mis-assembly of the SV40 promoter region.
Great work again. Forensically unraveling this mess piece by piece and so great to see this being done by different scientists across the globe, all validating and enhancing each others work. The language barriers will slow this down but the truth will out eventually .....
The technique to print the 72bp poly-A insert is called ribosomal frameshifting. It's limited to about 72bp, and is typically used for reporter, promoter or enhancer genes of small size.
The problems occur when the promoter sits in front of the wrong genes. For example, if it sits in front of C1ORF134, then there is a massive problem because that gene is a secretory peptide in the p36 cytogenetic band on chromosome 1 (p36 deletion syndrome).
Although removed from gene banks, C1ORF134 is listed as a sequence peptide in the patents, hence the use as an example.
Cells that received a DNA fragment with the SV40 promoter and the neomycin resistence gene (neoR/kanR) would produce the protein "neomycin-kanamycin phosphotransferase". This protein would be detectable by immunostaining or ELISA and possible antibodies produced by the patient would also be detectable.
The vaccination cannot be undone. Of course the immune system does its best to eliminate mutated body cells.
"The SV40 early promoter contains at least three spatially distinct elements: (1) two 72-bp repeats comprising the enhancer sequence; (2) three 21-bp repeats, each containing two (G + C)-rich motifs; and (3) the TATA box or Goldberg–Hogness box." https://www.sciencedirect.com/science/article/abs/pii/S0079660308603499?via%3Dihub
Keep going. I hear the groan of inevitability churning in the background!
What blood test would reveal presence & what counter for elimination ?
What are the implications of this? Cancers, I assume?
Great work again. Forensically unraveling this mess piece by piece and so great to see this being done by different scientists across the globe, all validating and enhancing each others work. The language barriers will slow this down but the truth will out eventually .....
Reverse Engineering of Pfizer jabs is wonderful!
Do you have a Molecular Weight calculator for the 72 bp insert, or any other interesting sequence of the string?
I would expect some gel electrophoresis controls here at the very least. Couldn't these just be artifacts of the assembler and tools being used?
The technique to print the 72bp poly-A insert is called ribosomal frameshifting. It's limited to about 72bp, and is typically used for reporter, promoter or enhancer genes of small size.
The problems occur when the promoter sits in front of the wrong genes. For example, if it sits in front of C1ORF134, then there is a massive problem because that gene is a secretory peptide in the p36 cytogenetic band on chromosome 1 (p36 deletion syndrome).
Although removed from gene banks, C1ORF134 is listed as a sequence peptide in the patents, hence the use as an example.
Cells that received a DNA fragment with the SV40 promoter and the neomycin resistence gene (neoR/kanR) would produce the protein "neomycin-kanamycin phosphotransferase". This protein would be detectable by immunostaining or ELISA and possible antibodies produced by the patient would also be detectable.
The vaccination cannot be undone. Of course the immune system does its best to eliminate mutated body cells.
"The SV40 early promoter contains at least three spatially distinct elements: (1) two 72-bp repeats comprising the enhancer sequence; (2) three 21-bp repeats, each containing two (G + C)-rich motifs; and (3) the TATA box or Goldberg–Hogness box." https://www.sciencedirect.com/science/article/abs/pii/S0079660308603499?via%3Dihub
The sv40 t antigen attaches to the oligomeric complex causing amyloidosis.