Introduction Using methods described earlier, we sequenced the Pfizer monovalent BNT162b2 vaccines and assembled these reads with Megahit. A 7,754 base pair spike encoded plasmid was assembled with a truncated Poly A tract and a previously described mis-assembly of the SV40 promoter region.
Great work again. Forensically unraveling this mess piece by piece and so great to see this being done by different scientists across the globe, all validating and enhancing each others work. The language barriers will slow this down but the truth will out eventually .....
The technique to print the 72bp poly-A insert is called ribosomal frameshifting. It's limited to about 72bp, and is typically used for reporter, promoter or enhancer genes of small size.
The problems occur when the promoter sits in front of the wrong genes. For example, if it sits in front of C1ORF134, then there is a massive problem because that gene is a secretory peptide in the p36 cytogenetic band on chromosome 1 (p36 deletion syndrome).
Although removed from gene banks, C1ORF134 is listed as a sequence peptide in the patents, hence the use as an example.
Cells that received a DNA fragment with the SV40 promoter and the neomycin resistence gene (neoR/kanR) would produce the protein "neomycin-kanamycin phosphotransferase". This protein would be detectable by immunostaining or ELISA and possible antibodies produced by the patient would also be detectable.
The vaccination cannot be undone. Of course the immune system does its best to eliminate mutated body cells.
"The SV40 early promoter contains at least three spatially distinct elements: (1) two 72-bp repeats comprising the enhancer sequence; (2) three 21-bp repeats, each containing two (G + C)-rich motifs; and (3) the TATA box or Goldberg–Hogness box." https://www.sciencedirect.com/science/article/abs/pii/S0079660308603499?via%3Dihub
"These data conclude that all Pfizer vectors contain a homoplastic 2 copy 72bp SV40 Enhancer associated with more robust expression and nuclear localization."
The concern, for me, is that it could be enhancing DNA alterations by CRISPR/CAS9:
"For Cas9 nuclease to exert genome editing activity, nuclear localization signal (NLS) derived from simian virus 40 (SV40) T antigen is commonly installed as genetic fusion to direct the intracellular Cas9 proteins to the nucleus of cells."
Keep going. I hear the groan of inevitability churning in the background!
What blood test would reveal presence & what counter for elimination ?
IF ELIMINATION POSSIBLE ..?
What are the implications of this? Cancers, I assume?
Yes. Lots. Metastatic.
Great work again. Forensically unraveling this mess piece by piece and so great to see this being done by different scientists across the globe, all validating and enhancing each others work. The language barriers will slow this down but the truth will out eventually .....
Reverse Engineering of Pfizer jabs is wonderful!
Do you have a Molecular Weight calculator for the 72 bp insert, or any other interesting sequence of the string?
I would expect some gel electrophoresis controls here at the very least. Couldn't these just be artifacts of the assembler and tools being used?
Have you read all the other substacks from February 16th, which detail the methods etc?
The technique to print the 72bp poly-A insert is called ribosomal frameshifting. It's limited to about 72bp, and is typically used for reporter, promoter or enhancer genes of small size.
The problems occur when the promoter sits in front of the wrong genes. For example, if it sits in front of C1ORF134, then there is a massive problem because that gene is a secretory peptide in the p36 cytogenetic band on chromosome 1 (p36 deletion syndrome).
Although removed from gene banks, C1ORF134 is listed as a sequence peptide in the patents, hence the use as an example.
Cells that received a DNA fragment with the SV40 promoter and the neomycin resistence gene (neoR/kanR) would produce the protein "neomycin-kanamycin phosphotransferase". This protein would be detectable by immunostaining or ELISA and possible antibodies produced by the patient would also be detectable.
The vaccination cannot be undone. Of course the immune system does its best to eliminate mutated body cells.
Not if the vaccine hits immune privileged cells.
Multiple studies show spike and nucleic acids persistence suggesting the immune system isn’t clearing these things in some people.
"The SV40 early promoter contains at least three spatially distinct elements: (1) two 72-bp repeats comprising the enhancer sequence; (2) three 21-bp repeats, each containing two (G + C)-rich motifs; and (3) the TATA box or Goldberg–Hogness box." https://www.sciencedirect.com/science/article/abs/pii/S0079660308603499?via%3Dihub
The 2 repeats From the study in the article:
"These data conclude that all Pfizer vectors contain a homoplastic 2 copy 72bp SV40 Enhancer associated with more robust expression and nuclear localization."
The concern, for me, is that it could be enhancing DNA alterations by CRISPR/CAS9:
"For Cas9 nuclease to exert genome editing activity, nuclear localization signal (NLS) derived from simian virus 40 (SV40) T antigen is commonly installed as genetic fusion to direct the intracellular Cas9 proteins to the nucleus of cells."
https://pubmed.ncbi.nlm.nih.gov/35200436/
The study did note that the T antigen was not found, but does not mention whether the NLS is present or not.
The sv40 t antigen attaches to the oligomeric complex causing amyloidosis.
It’s illegal to steal live inventory.
The analysis can only be performed on expired lots.
If they had nothing to hide they would allow access to the lots for testing.
Covidissonance: when qPCR is quantitative for viral detection but non-quantitative for vaccine detection.