Hi. I know nothing but I believe he means LNP when he says, "transfection reagent". I do not think potent is the correct word. Here's his article showing dsDNA packaged in the LNP:
I believe the important point is more that dsDNA encapsulated in the LNP crosses into cells. The FDA & EMA set limits for contamination assuming the DNA contamination would not be in the LNP and therefore unlikely to cross into the cells.
"Keep in mind these limits were set not anticipating the dsDNA to be packaged into a transfection reagent."
By "transfection reagent," you mean the LNP, which increase the potency?
Hi. I know nothing but I believe he means LNP when he says, "transfection reagent". I do not think potent is the correct word. Here's his article showing dsDNA packaged in the LNP:
https://anandamide.substack.com/p/lnp-packaging-of-dsdna
I believe the important point is more that dsDNA encapsulated in the LNP crosses into cells. The FDA & EMA set limits for contamination assuming the DNA contamination would not be in the LNP and therefore unlikely to cross into the cells.
Thanks!