The EMA documentation from Sasha Latypova discloses Pfizers linearization strategy which utilizes a TypeIIs restriction enzyme known as Eam1104i. Restriction enzymes cut DNA based on a specific sequence pattern. Eam1104i cuts at 5’ CTCTTCN^NNN 3’ on the top strand and 3’ GAGAAGNNNN^N 5’ on the bottom strand. This can be seen in the Pfizer provided vector map at base 4,280 or ~6:00 above.
This pucing tour is depressing but, nonetheless I pfind it pfascinating. Thank you for all you are doing. Miss being able to see soothing kitty pics on my feed to help me cope. When the world is melting down, there's nothing like a cat to reassure you life is still purrty great.
This pucing tour is depressing but, nonetheless I pfind it pfascinating. Thank you for all you are doing. Miss being able to see soothing kitty pics on my feed to help me cope. When the world is melting down, there's nothing like a cat to reassure you life is still purrty great.
Ben from USMortality failed to reproduce the genome of Wuhan-Hu-1 using MEGAHIT, but I think it might be because he didn't do any quality control or trimming or merging of the reads: https://usmortality.substack.com/p/sars-cov-2-genome-assembly, https://github.com/USMortality/Megahit-SARS-CoV-2/blob/master/megahit.sh. Are you able to modify his shell script so it produces the correct result?