Chimeric Spike-Spidroin transcripts?
Thank you Kevin. I pray that the world sees and understands the massive fraud taking place in real-time. Peace.
For the reverse strand ORF... it would be a substrate for 5' capping, but would lack the Kozak. So it seems chimeras as described above would be more likely to result in translated protein?
It would have the Kozak if generated from a 5’ spike that cis strand switched.
The capping is done after the T7 step so all transcripts will get capped.
The 3’ machinery would be missing from such transcripts (polyA).
Thank you Kevin. I pray that the world sees and understands the massive fraud taking place in real-time. Peace.
For the reverse strand ORF... it would be a substrate for 5' capping, but would lack the Kozak. So it seems chimeras as described above would be more likely to result in translated protein?
It would have the Kozak if generated from a 5’ spike that cis strand switched.
The capping is done after the T7 step so all transcripts will get capped.
The 3’ machinery would be missing from such transcripts (polyA).