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In PCR, primers don't really need to have unique genetic sequences, only PCR probes do.

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Thanks for the information. Seems like the bigger problem in the cannabis industry is that the majority of testing labs and assay producers don't post full validations for their hop latent viroid assays at all. Would be nice to see complete metrics, not just cross-reactivity, for each assay so they can be more easily compared to each other.

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I am just a bucket chemist. Give me a bucket and some chemicals and I am good to go. Back in the day we used PEG in polymer reactions as stabilizer for micelle formation in dual aqueous /oil phases. The monomer was "shot" with a redox agent causing propagation in the micelle. Nowadays they inject PEG into the bloodstream (lolz). But what would I know? I trust the experts. This looks like complicated shit to me. As far as I am concerned there are things we should not be messing with...the law of unintended consequences. The hubris of man. Give him a little knowledge....it is like giving a monkey a gun.

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Great write-up, thank you.

How difficult would it be technically to grab those target parts of human DNA, amplify them, and then keep a database about who has which DNA variant?

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OK, the virus exists, but "interactions" with bacteria killed people. https://doi.org/10.1093/infdis/jiab128

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