Brilliant comment - "Follow your principles and you’ll find yourself surrounded with people who understand doing the right thing attracts others that are in this for human freedom"
Yourself, Jessica Rose, a whole bunch of ex Saffers spread across the USA, Canada, UK (there are so many and you make me so proud of SA. This country produces great minds and critical thinkers - I've missed a lot but Kory, Marik, Lawrie, Hodgkins you are all amazing), Norman Fenton, Jikkymouse and his/her entire mouse army).You guys and Twitter have done more to expose MSM, the corruption within the scientific community and educating and keeping the public sane and safe than you'll ever realise. The way you've approached this has worn off on the people you've touched and the world is better for you. Sincerely, Thank you 🙏
Molecular genetics is not my strong suit, but looking into literature of SV40 incorporation into plasmids it is easily discoverable that genotoxicity studies had to seem vital to many overseeing the vax development. Referring to the SV40 72 bp tandem repeat as solely a promoter might be confusing since I envision promoters as a proximal sequence upstream of the gene. This might underlie the apparent lack of alarm by some as this sequence is not accompanied by the SV40 oncogene. The more complex function of the 72 bp SV40 element is generally referred to as an ENHANCER that is employed in transfection plasmids to greatly increase trans gene expression in all mammalian cell types. Here is my level of understanding: by binding multiple transcription factors, it functions as a DNA nuclear-targeting sequence (DTS) sequence to shuttle DNA into the nucleus and also increase expression of flanking genes (which can be up to 70 kb away). Any background info anyone can share to inform me on the relevant mechanisms is appreciated. BTW, here is a good chapter on the technology/concerns of enhancer elements in plasmids for gene therapy (esp insertional mutagenesis): https://link.springer.com/chapter/10.1007/978-94-007-4531-5_1
Is there reason to be concerned about gene mutation in human egg cells or not given their “static” nature (not a scientist so not sure what the right term is)?
Brilliant comment - "Follow your principles and you’ll find yourself surrounded with people who understand doing the right thing attracts others that are in this for human freedom"
Yourself, Jessica Rose, a whole bunch of ex Saffers spread across the USA, Canada, UK (there are so many and you make me so proud of SA. This country produces great minds and critical thinkers - I've missed a lot but Kory, Marik, Lawrie, Hodgkins you are all amazing), Norman Fenton, Jikkymouse and his/her entire mouse army).You guys and Twitter have done more to expose MSM, the corruption within the scientific community and educating and keeping the public sane and safe than you'll ever realise. The way you've approached this has worn off on the people you've touched and the world is better for you. Sincerely, Thank you 🙏
Molecular genetics is not my strong suit, but looking into literature of SV40 incorporation into plasmids it is easily discoverable that genotoxicity studies had to seem vital to many overseeing the vax development. Referring to the SV40 72 bp tandem repeat as solely a promoter might be confusing since I envision promoters as a proximal sequence upstream of the gene. This might underlie the apparent lack of alarm by some as this sequence is not accompanied by the SV40 oncogene. The more complex function of the 72 bp SV40 element is generally referred to as an ENHANCER that is employed in transfection plasmids to greatly increase trans gene expression in all mammalian cell types. Here is my level of understanding: by binding multiple transcription factors, it functions as a DNA nuclear-targeting sequence (DTS) sequence to shuttle DNA into the nucleus and also increase expression of flanking genes (which can be up to 70 kb away). Any background info anyone can share to inform me on the relevant mechanisms is appreciated. BTW, here is a good chapter on the technology/concerns of enhancer elements in plasmids for gene therapy (esp insertional mutagenesis): https://link.springer.com/chapter/10.1007/978-94-007-4531-5_1
Is there reason to be concerned about gene mutation in human egg cells or not given their “static” nature (not a scientist so not sure what the right term is)?
Thank you Kevin. I'm not transfected with contaminated gene-therapy crap; I guess I'd better get in touch with the clean blood company. Insane. Peace.
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