Sasha Latypova makes the case that it is technically impossible to scale up mRNA technology at present, and that AE variations in batches may be down to stratification in the vat, where all the broken fragments are floating in the nanolipid at the top, and the bottom consists of more inert material.
How can this now pose a risk to the unvaccinated? All "mutations" should carry enough of the prior sequence for our Immune Systems to identify and deal with the variant.
I am not a fan of open ended statements :-)
I also agree with previous post, that ALLOT of what was sent out were all really "duds". At the onset, the race to secure market share was so desperate that allot of the product might have been LNP blanks. There is also good research on LNP failure following freezing. So a large majority of deaths could be the LNP "sludge" that was injected.
If the VE is negative as seen in the Cleveland clinic study, that means you are more likely to get infected if you are vaxxed AND that also implies you are more likely to be generating variants.
2-5% of the population gets a reinfection from a CV annually. Increase the mutation rate and that reinfection rate will increase concordantly. So the unvaxxed/naturally immune will get another CV cold in their lifetime. Public health should not be using non sterilizing vaccines on a highly mutable RNA virus.
this will be a problem for the "at risk / vulnerable" portion of the population. the health immune systems will brush this off like a cold (as you said). But if you are old, frail and unvaxed. You will need some additional mechanism of boosting to make sure you dont get any deeper level lung infection.
Have you seen any deep-sequencing reads that indicate there has been recombination between the vaccinal sequences and the various SarsCov2 variants?
It looks like the codon optimised “pseudo-mRNA” vaccines match the Wuhan spike at around ~85% at the nucleotide level, with plenty of 30+ exact identity regions throughout the spike ORF to provide plentiful template switching possibilities, even for a +ve str RNA virus.
Various chimeras appear to have been found between delta and omicron after (presumably) a coinfection. And it even appears various entirely new viral species have been formed similarly, including possibly SARS Season 1.
Repeatedly transfecting millions of people who are potentially infected, with the reportedly far milder omicron variants, with billions of copies of modified nucleic acids that could recombine with any replicating virus to restore some (all?) of the linked mutations that made the original strain more virulent, seems like a risky plan to me.
As for a “bivalent” booster, containing the template for a recent but outcompeted omicron spike mixed up with the original Wuhan spike, being transfected into cells that may be replicating even more homologous omicron RNA, seems even more likely to promote recombination, and thereby provide an intermediate that could help restore the full Wuhan spike, than the original monovalent vaccine.
Is it known if the spike ORF’s in the bivalent shots were codon re-“optimised” differently to the original vaccinal spike to reduce the possibility of recombination with one another? I’m guessing not, seeing as the people who designed these seem to be oblivious to or in denial of a huge variety of plausible issues, as you have so comprehensively demonstrated.
Also, given the heavy codon changes in the vaccinal sequences, would the sequencing primers used for the transcriptome sequencing bind to the vaccinal regions if so? Or put another way, do we know if the routine deep sequencing has even looked for vaccinal recombinants? This amazing deep-sequencing was well after my time…
I would find it very ironic if some of the S gene amplimer “drop out”, that appears to still (bizarrely) be used as a diagnostic for certain variants, is actually coming about through vaccinal spike recombinants. Why continue to use a negative result as a diagnostic??
And lastly - sorry for all the questions in one post - is it known if the LFTs would give a positive signal for a vaccinal spike recombinant if it arose? If neither PCR nor LFT detects such a recombinant, then there would be a selective advantage to any such detection-evading variant that arose, and such cases might never even be sampled for the deep-sequencing needed to detect their presence.
P.S. Thanks for all the awesome posts (and to get even nerdier, particularly the deep dive into the modern usage of Type IIs REs, so much easier to say than hapaxoterministic restriction enzymes as I was taught to call them in the UK. I had some “fun” with them back in the day - took me right back to the early days of my molbio career.)
You raise an important question. Most Sequencing is being done with ARTIC primer pairs and such recombination events may be missed. The place to look is in random priming methods used in RNA-Seq data. There is now a lot of this data in the SRA. I have not had time to sleuth through it as Im still trying to find the Bivalent vaccine sequences. If anyone can find these, it would be a huge help. I checked the NEJM paper, the Clinical Trial docs in clinicaltrials.gov, the paper supplement, some FDA documents... Cant find them.
Sasha Latypova makes the case that it is technically impossible to scale up mRNA technology at present, and that AE variations in batches may be down to stratification in the vat, where all the broken fragments are floating in the nanolipid at the top, and the bottom consists of more inert material.
"Nobody Knows What is in the Vials" https://sashalatypova.substack.com/p/nobody-knows-what-is-in-the-vials
and that the DoD refers to the injections as "prototype demonstrations" https://sashalatypova.substack.com/p/the-role-of-the-us-dod-and-their
and that "Fake Western Blots Submitted by Pfizer to Several Regulatory Agencies" https://sashalatypova.substack.com/p/fake-western-blots-submitted-by-pfizer
"WHAT IS IN SO-CALLED COVID-19 "VACCINES"? Part 1: Evidence of a Global Crime Against Humanity" https://www.researchgate.net/publication/364819531_WHAT_IS_IN_SO-CALLED_COVID-19_VACCINES_Part_1_Evidence_of_a_Global_Crime_Against_Humanity
---------------------------
There are 52 covid vaccines in clinical trial in the US alone, 29 of which are mRNA/DNA https://covid19.trackvaccines.org/country/united-states-of-america/
And, in what I'm certain is merely a coincidence- "MRNA Cocktail Can Make Old Tissue Specific Cells Young" https://www.nextbigfuture.com/2022/03/mrna-cocktail-can-de-age-cells-and-retain-tissue-differentiation.html
A very interesting read!
Great post Kevin. You're writing's gotten clearer too
How can this now pose a risk to the unvaccinated? All "mutations" should carry enough of the prior sequence for our Immune Systems to identify and deal with the variant.
I am not a fan of open ended statements :-)
I also agree with previous post, that ALLOT of what was sent out were all really "duds". At the onset, the race to secure market share was so desperate that allot of the product might have been LNP blanks. There is also good research on LNP failure following freezing. So a large majority of deaths could be the LNP "sludge" that was injected.
If the VE is negative as seen in the Cleveland clinic study, that means you are more likely to get infected if you are vaxxed AND that also implies you are more likely to be generating variants.
2-5% of the population gets a reinfection from a CV annually. Increase the mutation rate and that reinfection rate will increase concordantly. So the unvaxxed/naturally immune will get another CV cold in their lifetime. Public health should not be using non sterilizing vaccines on a highly mutable RNA virus.
thank you for replying. Most appreciated.
this will be a problem for the "at risk / vulnerable" portion of the population. the health immune systems will brush this off like a cold (as you said). But if you are old, frail and unvaxed. You will need some additional mechanism of boosting to make sure you dont get any deeper level lung infection.
Hi Kevin,
Have you seen any deep-sequencing reads that indicate there has been recombination between the vaccinal sequences and the various SarsCov2 variants?
It looks like the codon optimised “pseudo-mRNA” vaccines match the Wuhan spike at around ~85% at the nucleotide level, with plenty of 30+ exact identity regions throughout the spike ORF to provide plentiful template switching possibilities, even for a +ve str RNA virus.
Various chimeras appear to have been found between delta and omicron after (presumably) a coinfection. And it even appears various entirely new viral species have been formed similarly, including possibly SARS Season 1.
Repeatedly transfecting millions of people who are potentially infected, with the reportedly far milder omicron variants, with billions of copies of modified nucleic acids that could recombine with any replicating virus to restore some (all?) of the linked mutations that made the original strain more virulent, seems like a risky plan to me.
As for a “bivalent” booster, containing the template for a recent but outcompeted omicron spike mixed up with the original Wuhan spike, being transfected into cells that may be replicating even more homologous omicron RNA, seems even more likely to promote recombination, and thereby provide an intermediate that could help restore the full Wuhan spike, than the original monovalent vaccine.
Is it known if the spike ORF’s in the bivalent shots were codon re-“optimised” differently to the original vaccinal spike to reduce the possibility of recombination with one another? I’m guessing not, seeing as the people who designed these seem to be oblivious to or in denial of a huge variety of plausible issues, as you have so comprehensively demonstrated.
Also, given the heavy codon changes in the vaccinal sequences, would the sequencing primers used for the transcriptome sequencing bind to the vaccinal regions if so? Or put another way, do we know if the routine deep sequencing has even looked for vaccinal recombinants? This amazing deep-sequencing was well after my time…
I would find it very ironic if some of the S gene amplimer “drop out”, that appears to still (bizarrely) be used as a diagnostic for certain variants, is actually coming about through vaccinal spike recombinants. Why continue to use a negative result as a diagnostic??
And lastly - sorry for all the questions in one post - is it known if the LFTs would give a positive signal for a vaccinal spike recombinant if it arose? If neither PCR nor LFT detects such a recombinant, then there would be a selective advantage to any such detection-evading variant that arose, and such cases might never even be sampled for the deep-sequencing needed to detect their presence.
P.S. Thanks for all the awesome posts (and to get even nerdier, particularly the deep dive into the modern usage of Type IIs REs, so much easier to say than hapaxoterministic restriction enzymes as I was taught to call them in the UK. I had some “fun” with them back in the day - took me right back to the early days of my molbio career.)
You raise an important question. Most Sequencing is being done with ARTIC primer pairs and such recombination events may be missed. The place to look is in random priming methods used in RNA-Seq data. There is now a lot of this data in the SRA. I have not had time to sleuth through it as Im still trying to find the Bivalent vaccine sequences. If anyone can find these, it would be a huge help. I checked the NEJM paper, the Clinical Trial docs in clinicaltrials.gov, the paper supplement, some FDA documents... Cant find them.
Fascinating and great detective work! Thank you.
+1 for #FiatScience...I'll be spreading that one on #gab.