Sequence of the Janssen/J&J Ad26.COV2.S DNA vaccine.

Figure1. SnapGene annotation of the 33kb Janssen/J&J vaccine sequence. Spike is in red. Predicted ORFs from 6 frames are in Yellow and Green. MGC Spike Primers and Probes annotated in Purple.

Introduction

We sequenced a DNA isolation from an expired Janssen/J&J Ad26.COV2.S vaccine. Included is the assembled sequence, BAM file and SnapGene annotations file.

Lot information.

>JJ_Ad26.COV2.S_k141_352

CACAAAGTAAACAAAAGTTAATATGCAAATGAGCTTTTGAATTTTAACGGTTTTGGGGCGGAGCCAACGCTGATTGGACGAGAAACGGTGATGCAAATGACGTCACGACGCACGGCTAACGGTCGCCGCGGAGGCGTGGCCGGACATGGAAGCCATTTTGGACGTGGGCAAGATGGCGCCCTACGGCTGAGTTACATGTAAGGGCAGAAGTGACGTAACGTGGGAACCCGGAAGTTGAGTCACGAAATCGATACGAGGAAGTGAGAACGCGGAAGTGTTTTCGCGAAAAACACCCAGGGCGTGTGTGTTTTTTTGGCGGGGTTTTTAGACCTTTGGTCATTACGTCAGTGTAAGGTGCCTTTAAATAGGGAGCTGGGTCAGTGGGTAGTCAGTCGCTGCTGTTGGCGCTTGTACTGCTGTGCTCTCTGTCTACTGGTAAGCGGGCTGAGCTATGGCTGAATCTCTGTATGCTTTCATAGATAGCCCTGGAGGGATTGCTCCCGTCCAGGAAGGGACTAGCAATAGATATACCTTTTTTTGCCCAGAATCTTTCCACATTCCTCCGCATGGGGTGGTTTTGGTTCACTTGAGAGTGAGCGTGCTGATTCCTAATGGCTACCAAGGCAGATTTATGGCCTTGAATGACTACCATTCCAGGGGCATACTAACCCAGTCCGATGTGATCTTTGCTGGGAGAAGACAGGAGCTAACTGTGCTGCTCTTTAACCACACGGATCGATTTTTGTATGTCCGAGAGGGCCACCCAGTGGGCACTTTGCTGCTGGAGAGAGTGATTTTTCCTTCAGTGAGACTGGCAACTCTGGTTTAGTGCATGCTTTCTGTTTTTCAGACTCTCATCTCTACTAACCATGCTTGAGCGACACGCTGTCAGCTACCACATTGTGGTTCCTGGGGTCCTAGTGACTTATTTAGAGGACTTTTCTATTACTCAAATGATTAAAGAAAAACTACCTCGCTTTATCACTCACCTATTGGAAGGCATCACCGGTGACACAAAGAGAGCTTATTCCAGCATGCAGTTTTTGGGTGCTAGCTTTGGAGCATTAAAGTACTCACTCACGCTTGCCAGTCCCACGCTTAGCCCTGGCTCTGAATTGTCAGCTGTAGTGGCTGAGGACTTGAGTGACTTTTTGCAGCTGACGCTGAGACGTGAGCTGAGGGCAGAGGGCAGAACCTCATTGAATCTTGTTGTTTTGAACACGCTGCAGGTGGTGGAGCAGCAGGATCTGTTTCTAATATGAAGGTCTGCCTGATTATGAAAGTGGAGGGGGCGCTGTGGGAACTTTTCCACATGTGCGGAGTGGACTTACACCAACAATTTGTAGAAATAATCCAAGGCTGGAAAAATGAGAATTACCTGGGGATGGTGAATGAGTGTAACCTGATGATTGATGAAATCGATGGAGGTCCAGCCTTTAATGTGATTCTTATGCTGGATGTACGCGTAGAGCCACTGCTCGAAGCCACAGTCGAGCACCTTGAAAATAGAGTGGGTTTTGACCTGGCTGTCTGTTTCCACCAGCACAGCGGCGGGGAACGCTGTCACCTGCGTGACTTGCACTTTATCGTCCTTCGAGACCGCTTGGAATAAAAATGGTTCTTCCAGTTCTTCCCCCACCTCCTCTTCATGATAGATCAGGATGCATTAACTGGATTGGGATGGCCTACAATGTCCTGGCTGATGTTTTAAGGGGAATCCGCATCGACGGGACTTTTGTTTCATCAGATGCACAGGAGCTGCTGGAGAACCTGCGGGAATGGCTGTACTTCAGTTGGATGACTGAGCGTCAGCAGCGGAAGGACAGCAGGAGGAAAGGTATCTGCTGTTCCCGAGCCACTTTCTGCTGGCAGAAGTACGACAAGGTACGCAAGAGAATCCATTTCAATGACTACGTCCGGCGTTCCATTTGGCATGACACTACGACCAACACGATCTCGGTTGTCTCGGCGCACTCCGTACAGTAGGGATCGTCTACCTCCTTTTGAGACAGAAACCCGCGCTACCATACTGGAGGATCATCCGCTGCTGCCCGAATGTAACACTTTGACAATGCACAACGTGAGTTACGTGCGAGGTCTTCCCTGCAGTGTGGGATTTACGCTGATTCAGGAATGGGTTGTTCCCTGGGATATGGTTCTAACGCGGGAGGAGCTTGTAATCCTGAGGAAGTGTATGCACGTGTGCCTGTGTTGTGCCAACATTGATATCATGACGAGCATGATGATCCATGGTTACGAGTCCTGGGCTCTCCACTGTCATTGTTCCAGTCCCGGTTCCCTGCAGTGTATAGCCGGCGGGCAGGTTTTGGCCAGCTGGTTTAGGATGGTGGTGGATGGCGCCATGTTTAATCAGAGGTTTATATGGTACCGGGAGGTGGTGAATTACAACATGCCAAAAGAGGTAATGTTTATGTCCAGCGTGTTTATGAGGGGTCGCCACTTAATCTACCTGCGCTTGTGGTATGATGGCCACGTGGGTTCTGTGGTCCCCGCCATGAGCTTTGGATACAGCGCCTTGCACTGTGGGATTTTGAACAATATTGTGGTGCTGTGCTGCAGTTACTGTGCTGATTTAAGTGAGATCAGGGTGCGCTGCTGTGCCCGGAGGACAAGGCGCCTTATGCTGCGGGCGGTGCGAATCATCGCTGAGGAGACCACTGCCATGTTGTATTCCTGCAGGACGGAGCGGCGGCGGCAGCAGTTTATTCGCGCGCTGCTGCAGCACCACCGCCCTATCCTGATGCACGATTATGACTCTACCCCCATGTAGGCGTGGACTTCTCCTTCGCCGCCCGTTAAGCAACCGCAAGTTGGACAGCAGCCTGTGGCTCAGCAGCTGGACAGCGACATGAACTTAAGTGAGCTGCCCGGGGAGTTTATTAATATCACTGATGAGCGTTTGGCTCGACAGGAAACCGTGTGGAATATAACACCTAAGAATATGTCTGTTACCCATGATATGATGCTTTTTAAGGCCAGCCGGGGAGAAAGGACTGTTTACACTGTGAAATGGGCTGGTGGTGGGAAACTGACTACCCGTGCTGGTGTAAAAATAAATAAAGATTCATAAATTCAGTTCAAAACATTTTATTAGTCTTTGTCTTTGTTCTCTTGGGTAAGATATGAGAAGGTAAAAGATGTAACATCAAACTGTTGGGCAGAATAGTCTTTTTGCCAAGTGAAGGTAAATGTTATAGAATAAGCACATCCATTTTCAGTTTCCTGATTAAATGCAATAGTGATAATTACAGGTTGGTAGGTAAGAGCACCTAAGTAGACATTACTTATGATTTTAGTTCTTGAATAGTTTTTTGCATTTTGAGCGCTTGGTTTTGGATAAGCTGTCAGGTTTGGCATGAATGGAACTGCATTATTATATTTTTGAGATACAATAGAATCATCACTTCTGTAATTCCAATAATCTTTGCTAAGGGACGAAGATGGTAATAGAATTCCATTCTCATCAAATAGTAAAGATATTTTTACATCCTTCTTTGTAGTAGCAGTCATTTGATGATATTCACCTGTAACTGCAAGTAAAGATACATTTCCCAGAACCTGACTTCCACACTTTGTAAGTGTCAACGTAAGTTTAGAATCCTTTTCTGTACTCATTTTGCAATTTGGAGATGTGTCAGGGGTTGTCCACAGGGTGCGCCTGTCATCTTTTTTATTCCAGGCTACTAAATCACCCTTTTTATCAAATGTCAGCCCGCCGTCATCTCCAAGTCTTACACGCAAGCCGACTCCTTTGTTTGTTACACCATCGTCATTTTTTAATTCCTCCGTTCCTATTCCCCTTCCAGTTAATACCACAAGTGTATTCAATAATCCAGTCAATCCAGTAATGGAATTATCTAGTACTTTTAATCCATTACCTGCCTTTAAAGCAAGCTTTCCATCTCTGACCTCGAATGGAGGAGCCAGAGCAAGCTGTAGCTTATCACTTGCAACTTGCAAGGGTGCATCAGGGTTTACGCTTAGGTTACCAGTCTGTTGCTCTACAGCAAGTCCCCCTCCCACCTTAAGTGAGACATCCCCATTGTTGATGGTGATTGGATCAGCCAGTTTAAGTGACAGGACCCCAGGGGGGAAGTTTTTGAATCCATCGGAGGAGACAAAGGGGGGAGTGAGGAAGGGGATATTCTGATTCCGCGCGTAGCCATAGGGGTAGACGGGGTTGAAGTCATCTTCCACCCGGAGCCTCTTTGCCATCTGAGAGGGAAGACAATGAAAATTGTGGACCAGGAATTTGACATCCCTTTCAAGGTGTGGAGGAAGTTCGCCGCCCGCCGGGGACTGGAGTACCAGAGCTGGGAGGAGGGTACCGAGGTGCTGCTGAACAACTACACCAGAGACATACTTTCAGATTTCAAGTAAGTGATCTTTATTGAATGATTAATTCCAATGATTTATTTTTTATGAGTAATTGGGGGATGGGGAAATGGGGGAAGGGGCTGATTGGTTTTTGGGCTTTTAATCAACATCAGTTCATGGACTAGTGCAGTGAGTGTTGGAGACTGCAAATGAGGTGTTCTCGAAGAACCAGATGATATAGGTCTTGTGTGGTCTGCAGGTGAGTTTTACCTCAGTCTGGTTGGTGAGGTAGACGGTCTCGTTGAGTTGACGACGACAAGGTCCAGAGTATTGCAAGAGTTTAGCTTTTATTAAACTCACCACAAAGAGTACAACAAAAATTCTCATGCAACTTCGGGGTTGGACAGATGCAGTAGACGGTGTAGGTGGTGGCGCCCCGGTCCCGCTCGACCAGGAAGGGTTGCTGGGTGAAGAACCTGACCAGGTGAGACTCGGTGGTGCCCTCTACGGTGATCCGCACTCCGTGTGCAGGGCCGTTGGGGTGCTCCTCGGGAAACTCAAAGTAGATGAGTCCGCAGCTCTCCCGGGCGAAGCAGCGCAGGCGGCGGCAGTGGTCCAGATGCCTCAACCGAGCGAGCTCAGCCGAGTCACCATGGGACATTCAGTCGTAGCCGTCCACCGCTTCTCTCACTGCGTCGAAGTTCGGTATGAACTCGTCCGGGTAGAGGCCTGGCGATCCCGAGAAGGGGTTGAAGTAGACCGAGGGCACAAACTCCTCCACAAACTGGAGGGTTCCGATGCCTCCGGAGCGAGGCTCCGAAGAGCTGCTCTGCAAGGTCAGGTAGGCCTGGTTGGGAGTGAAGGAGGATCTCCCGGCTCCGGCTAGTTGGAACACTCCGTCCGGTCGCAGACCGATCGAAGAGCTCACCAACTCGTCGTTGAGCTGTGTGCCTCTGCCTCGGATCACCAGGGTTTTTATACCCGATTGTGGGCGGAGCGGGCACCGGGAAGCGCCGCCGGCCAGCTGGACACCTGAGTTAGTCATGCGGACTTCGGCCAGTGCGTCACGCGGAAGTAGTACGGTAGTCGGCCCGGGGATTTCCTGATACACCAGGGTGGAGGGCCAATTACGCGGGTTGAGCTTTGCCCTGGGCGTGGAGGTGACCGCCGCCTGCTCCAACAATATCTGGTTTCGATGACTGCGGACCCCGTTGACCCGTGAGATCATCGAGGGGCCGGCACTGAGCCAGTTCATGCGGGTGGAGTAGTCCTGGGAGGCGCCCGCGGCCAGGCCCATTTGGGGCTGATAGCTCCACATGTAAGGGGTGGGAATCTCCTTGCTCATGGTGGCGATGAGGTAATTCCCGCCTTTTTTCCGGGTGGGCGCGCCTTAGTCTTTAAGAGTGAGCGCGCAGTACTTGTGGAACAGAGCCTCGGCGTCTTCGAGAGTGCGCTGCAGCTGATCTTCGTTTTTGTGGTACAAGCAACTGCGGGTGAGCGAGCGCAGAGACCGGTTTTTTACTTTGAGCTCTTGCTGCTGACCTCGACTCTGCTGAAAAATGGCATAAAGAGTGGGGAAGATCCGGTTCCTCAGCTCCCTGGTGGTCAAGGGCTCGTTCGCCGCGATCCTCAGGCCTCCTCCTCCTCCTCCGGCGACTCCTTTGACTTGCTTTTTCTTAGCTGTGAAGGTGACGGTAGTAATGCAACACGTTGCGGGGGATGTTCACCCCGCGGTGGAAGAGCAGGTAGCGGCGGGCGAAAGCGATGTTCCCCCCGCAGTCTTGCAAGCAGGCGATGATCGCGGCTTTGTGGGCCCTCCATGATCGGTAGCCCTGCCGCTCGTGCTTACCGTCACCCTTCACTTGATCCCATCTACGAGGAGGCTTGACCGGAGCGGAGGTTGTATCCGTGACCGGCGGGGCTGCCGGCGCGGATGGTGCGGCGACGGGCTGCTCTGTTGCCTGCTCCTCTTCCACCTCTTCCAATTCTTCCTCCTCTGTCTCCTCCTCCCATTCGTCCTCCTCTGCCTGGCTGTCCCATTCTTCTTCCATCTCCTCCACTAGCGGCTCCTGCTTCTTCCTCGGCATGCTGGGGGAGACCCGGGTTGAGCACCTCGCCCGTCTGGGGGTCCAGGTAGACCCCTCTACCCTTTTTCAGAAGAAACTCTTGGCGGGATTTTTGGATGGCGTGCAATTGGGCGAGGATCGCGCCCTGGGTGATGACGCAGGCCGTGAGATCCGCGTGCTGCGGACGCGATTGGTCCTCAAAGAACTTTATCTCATGGGCGTGGTAGTCTTCGGGTACAAATTTGCGCAGGTACGCGGAAGTCCACAACCCCGGCGTGAGTTTCAGCGGAGCGGTGGACTCCTGCGGACCTTGCAGCTCGAAGGTACCGATGACCTGGGTCTCGCTCAGGAGCTGGGGGTTGCAGACCAGGGAGCGATGCGGGGAGCACAGGTTGCAGCGACAGTGGCACTCGAGCAGCCCCTCGCCGCTCACGTCCTCGATCACGTCCGAGTGGTAGGCGATGTAGTTGGCCAGCTGCAAGAGGTAGCAGTGGCTCCACAGCGGCGGGGGACACTCGCGGTAGCTCAGCGGTACAAAGTCGGAGGGGAAGGCGTTGCAGGTGGCGGGCAGGATGCCTGATCGCTCAAGAATGAAAGTGCGGTAGTTTTGCAACATGCTCTGGCTCATGAAGTCGGGCAGCCCGCCTTTCAGCGTCTGTCTCAGGCGCTCGGGGAAGACGATCTCGGCCAGGTCCGAGGCGACGGTGCGCTCGTCGAAGCCCGTCCACAGCTTTTTAAGGTTTCTAGCAAGAAGCTTGTCCAGCTCCTTTAGGTTCTCGTCCTCGAGACACTGCTGCCACACGCCCATGGCCGCTTGCCATGTGTGGCAGAGAAAGAGAAAGACGCAGTCGCGGACATAGTCCCGCCGCGCCTCGCCCTTCAGGGTAGAGTGGAGCACGGTCTGGCCCAGGCGGTTCTCGTGCAGGATCCCCAGGCAGGAGACCAGGTTGGTCAGCTCCACGTTGGAGATCTTGCAGGCCTGCCTGACGAAACCGTGCCTGAAAGTGTAGTGCAGGGTCTCCTCGACCTTGCGCAGGGTCTCGGGGTCGCTGAAAAAGCGGCGCATGCATTCGAGCTCAAGGGTGACCAGGACCACGGCCAGCATGAGTTTGCGCCGCTGTTCCAAAGCCTGGGGGTCGCGGGTCCCGAGCCAGCGCGCGAGCATCTCGTCGCTGACCACGGGCCGGGCGTCCTCGGGGCGCTGCTCCTGACTTTCATCGAGGGCCTCGATGTGGGGCATGATGAGCTGATCCATGATGCGACGCATGACCTTGGGCGGGAGGTTGACGGCGGGGTAGGCGAAGTGGGTAAGCTCGACGCTGCGCTTGAGCACGGCCAGCCTGGCGTTGTCGCCTTCCAACTCTACCAGGGCGCTAGTGTGACCCTCTTCCTCTGCTGTTTCTTTCAGAGCGTTCGCCGCGCGCGTCTCGTCCCGACCGAGCCCTTCGAAGATCTTGGGCACCTCTTCCAGGGAAGCGATATCAGGTATGCGCGCGCCGGGCCCCAAAGCGAGGAGCGCGTCGGCGCTGGTGCGGTTGGCGCGGCAGGAGACGGGGATCTTTTGGTTCTTGAAAAAGATGTGATAGGTGGCGAGGGCTTCGGGGACCGCAAAGACGGGATAGAAGTTGAGACGCGGGTTGGGCTCGCAGGTGCCGTTGGGCTGGCGTTTGGGGGGTACGCGCGGCGAGAAGAGGTTGAGCTCGTAGGCCCGACACAGCTCCTCGACGCTGAGGGGGGTTTCGCTCCGGTCGGCCAGGGCGTCCCGGAGGATGAGGGACTGGCGCTGCAGGTGTTTCAGCAGCACATCCTCCTCTCCTCCCAGGTAGCCATGCTCGAGCCCAGCGTCGGTCTCCTCCTGGCCTGCGTCTTGCTCGTGCTCCTGTTCATCCTGTGGGGGTTCTAGACGAGCCGGCTCTTCGAAGCCCAGGTCCTGCTCGATTTTGGGTTGCTCGTGGGTGGTTAAGTCCTCCTCCTGCTCCTGCTTACGCGGCTGCTCCTCCATCTTCCCCTAGGAAACAATGGCCGGCGGCAGTCAGGACGTGCGCCGGTTCATGGGCCGAGAGGCCACTCCGCCCCGGGGCCACGGGTCGGCGCGCTATCCGCCGGAGCAGGAGAGGAGCCCCTCGCCGCCACCTCCTCTGCCCACCAAGCGCCGAAAGTATCAGCGGGTGGGCTCCGGGTCTTCCGAGGAGGACGTGCTCCCCGTGGACAGCCCTCCAAAAAAGAAGCAAGCCAGAAAGACCAAGCATGTGACCAAGGTAGACCCCGACGAAGAGATGCCCCAGGAAGATGCCGTGATCGTGGGAGTGGGATTCAGCCAGCCGCCGGTTCTATTGAAGGAAGGCAAGGACGGAAAACGCATCGTCGAGCCCGCGACCCCCGGTGTCCTGAACGTACGCAACCCTCTGAGTCTGCCTCTGGTCTCGTCCTGGGAGAAGGGCATGGATACCATGAACGTGCTGATGGAACGCTACCGCGTCGACAGCGGCCTGCGCGATGCTTACAAGCTCATGCCCGAGCAGACCGAGATCTTCCAGAAGATGTGCCAGACCTGGATGAACGAGGAGGCCCGCGGGATGCAACTGACCTTCACCACCCAGAAAGCATTTAGCACCATCATGGGCCGCATGTTGCAAGGTTACATCTTCAGCCACAGCGGGATTGCGCACAAGAACTGGGAGTGCACCGGTTGCGCCCTGTGGGATCACGGCTGCACCGAGGTGGAAGGCCAGCTCAAGTGTCTGCACGGGACGATGATGATCCACAAGGACCACGTGGTGGAGATGGATGTGACCAGCGAGAACGGGCAGCGCGCGTTGAAGGAGCAGCCCAGCAAGGCTAAGGTGACCCAGAACCGCTGGGGACGGAGCGTGGTGCAACTGACCAGCCATGACGCGCGCTGCTGCGTGCAGGATGCCGGGTGTGGGAATAACCAGTTCAGCGGGAAGAGCTGCGGCCTGTTTTTCAGCGAGGGGGCCAAGGCCCAGCAAGCTTTCAAGCAGATCGCGGCCTTTGTCAAGGCCCTCTACCCGAATATGCAGCGCGGCGCGGGGATGATGCTGATGCCCGTGCACTGCGAGTGTAACCACAAGCCTCAGAGCGTGCCCTTCCTGGGACGCCAGCTGTGCAAGATGACCCCGTTCGGCCTGAGCAACGCCGAGGATCTGGACAAGGATCAGATCAGCGACAAGAGCGTGCTGGCTAGTGTGAAGTACCCCAGTCTGATGGTGTTCCAGTGCTGCAACCCCGTGTACCGCAACTCGCGCGCGCAGAGCACCGGCCCCAACTGCGATTTCAAGATCTCCGCCCCGGACATGCTGGGCGCCCTGCAGATGAGCCGGCGCATGTGGAGCGAGACCTTCCCCGAGACCCCAGTGCCCAAACTGGTGATCCCCGAGTTCAAGTGGCAGCCCAAGTACCAGTACCGCAACGTGGCCCTCCCCAGCGCGGCGCACAACGACGAGCGCGAGAACCCCTTCGACTTTTAAATAACTTGCATATACTCCAGTGCATGTAAAATAAAAGTGCTGTTTATTGAGATACACGCAGTTGTTTCATTTTGTCAAAAGCGGTGGCGTGTTCGATGGCGGCGCGGTGGGAGCGAAAGTAAGGGGAGTGGCGCGCGAGGAAGCGGTAGAGTTCCTCCTGGTTGCGCCTGAGGGTGGGCAGCACCTGTGGCGATTGTAGCATGCCGTTGGGCACCCCCGTCAGCAAGTTCATGGTGGGGTTTCCGTCCATGGGTCGGTCGGGCCAGTGCACGAAGGCATGCAAGAACATGCAACAGAAGAGTCCGCAGGCGGCGGAGTCGGGCCCCTGCACGGTCTGAGTGGACTGCTCGAGGCTGAGACAGCGGTCGGGCGAGGAGGCCAGGGCGCTGCGGCGCAGCATGGCCTCGTACTCGAAGCTGTAAATCTGCTTGAGCCGGCGGTCCGAGAACCCAAAGGGGTCGAACATGTAGCAGGTGCGCGAGCGCGGGTTCCAGCCGAAGGCGAGCCAGTGCACGCCTCCGGTCTCGCGGCCGGCCGTGTTGACGATGGCGCAGGCCAGCTTGTCGCCGGCGAGAAAGCCCGGGAAGCGCTTGTCGTGGGTGCCCAAAAAGTAGGGCCCGCAGCCCAGGTCGCGCACGATGGCCGCGAGCTCTCGTTCGCTGGAGCCGCTCATGCTTAGGTGGTGGCGTTGCCGGCGGAGAAGGGCGTGCGCAGGTAGACGGCCTCGATGACGCCGCGGTGCGGCTGGTGTACTCTGACCACGTCGAAAACTTCGAAGAGAAGATAGAGGAGGGTGGGCTCATCCATGGGGTCCACCTCGAAGGTCATGTCGAGCGCGTGGGCCGAGTTGGCGTAGAGCATGTTCTGACCCAGGTCGGTGAGGGCGCCCATGGACATGAAGTTGCTGGAGAAGGGGATGCGCCACATGACCCTGTCGCAGAGGAACTTTTTCTGGGTGACGGAGGGCACGGCTGTCTGACCGATGAGCGGGTAGGGGAAGTTGGCGGGGTAGGGCTGCCCCTGGCGCATGGTGGGTGCGAGGTAGCCGGTGAAGCCCGAGTTATTGTGCTGGAAGGGCAGGGTGACGGCCTTGTAGTCCTTGTAGTTGATCTCATCGACCACCTGCCTGCTCATGGGCTGGAAGTTGCGGAAGAAGGAGTACATGCGGTCCTTGTAGCCCTCGGGCACGTGGAAGCCCTGGTAGCCGATGTTGTAGTGGGAGAGCATCTGGACGAGGAACCAGTCCTTGGTCATGTTGCATTGGGCCACGTTGTAGCCCTCCCCGTCGACGCTGCGCTTGATCTCGAACTCGTTCGGCGTGAGCAGCCGGTCGTTGCCGGGCCAGCTGACCGAGGAGTCGAACATGATGGAGACCTTCTTGAAGGTGTGGTTGAGGTAGAAGGTCCCGTCGAGGTAGGGGATGGAGCCCGAGTAGACAAAGTAGGGGTCGAAACCCGAGCCGAGGGAAGGAGTTTCCTTGGTCTTGAGCCGGGTGAAACTCCAGCCGCGGAAGGCGGCCCAGTTGCGCGATGGGATGGAGATGGGCACGTTGGTGGCCTTGGCCGGGATGGGGTAGAGCATGTTGGCGGCCGAGAGGTAGTCGTTGAAGGACTGGTCGTTGGTGTCGTTGCGCAGCATGGCTTCCAAGGTTGAAGCGGTGTTGTGCGCCATGGGGAAGAAAGTGGCGTATAGGTTGACGCTGTCGAAGCGGACGGAGGCGCCGTCGACGCGCAGGTCGTTGCCGAGGGAACTCTGCAGGATCATGTTGACGTCCTTGCGGAAGTTCCACTCGTAGGTGTAGGAGCCCGGGAGCAGGAGCAGGTTCTTGATGGCAAAGAACTTTTGGGGCACTTGGATGTGGAAGGGCACGTAGCGGCCGTTGCCCAGCAGCATGGAGCGGTAGCGCAGGCCCGCATTGCGGTGGTGGTTGAAGGGGTTGACGTTGTCCATGGGGTCCAACGACCAGCGGGCGCCGATGTTGATGTAGGCGTCCACCAGCGAGGGGGCTACCACGCGGCCGTTCATGTACTCGTAGGTGTTGGTGTTGGCGGGCAGCTTGACGTTGGCCGGCGTGTACTTGTAGGAGTCGGGCAGGTACAGGGCCACGTTCGAGTACAGAAAACTCTTCCACAGGTTGGCCTGCAGGTTGATCTCCATGGCGTAGACATTGCCCTTGCAGATTTGGTTTTGTCTAGAAATTGCATCGTCTTTCTCCCACTCACTTTCTTCAGCACCATCATTACCATTTGTAATCTTTACACCTTGATAAGTGGAATTGGTTCCAGTGCCATTCAATGGGAAGCAATAGTTTGGAAGTTCATCTTCCACACCGTGATTTTCAATGATCCTGACATCTGGATCGTAACTGTCCACCGCAGAGTTCCACATGCTAAAGTATCTGGTTCTGTCACCCAGAGAATCTAGCAATAGCTGGTAAGATAACTCGGTGTTTCTGTCTTGCAAGTCGACCACAGCGTTCAACTGAGAAGCCTGACCAGCCAGCACACCCATATTTCCGGTGCTGTTGTAATACATGAGACCTACAAAGTTGTCCCTAAAGCCAATGTAGTTGGGTCTGTTTGGCATGGACTGCTGAACCAGATTGATTTCTGAACTGTTATCTGAAGTTCCTGGCTTGTAAACCACATGAGTGTCTGGTGTTTCAAGATTAACATTTTCAGTGTACAAAATTATATCTGCTTTGTATTCTTCCCCACTACCACCTGCTGGAGGACTTCCGCCAGGGACGTCAAAGTAAGCAAAATCTATATCCAGATCTTTAGGTTGTTCTCCTTCATTAACTGGTTTGAACTTTGCCTGACCTCCTTTCTCATTAGTAGGTCTAGCAAAAGATCCATAGCATGGTTTCATTTTAGTGTCCTTTTTAAGAGCCCTTCCTCCATAGAAGGCTTCATTTTCCTGCCAGTTTTCTTCTCCAACTTGAGGTTCTGGCTGGAAAGTCTTGTCTGCATAAATGTCTTTTTTGCCATTCTCAGCGGTTTCGTCAGTTCCTAGTAACAGACCCTGGTTTGTTATATTAATTCCGCCGGTGGCAGCCACACCAAATGTTTTTGTGACATCTTTTTCTTGCTGCACTCCTCCAGTAGTTCCTTGCTTTTCTTTTGTTTCCCACTGACTGGGATTGGGGGCACCCTTGGGAGCCAGGCTGTTGTAGGCCGTGCCCGAGTAGGGTTTGAAGCTGGGACCGCGGTCCAGGACGCCGCGGATGTCAAAGTAAGTGCTGGCCATGTCTAGCACCCGGTTGTCGCCCACGGCCAGAGTGAAGCGCGCCTTGTACGAGTACGTGGTGTCCTCGCGATCCACGGGCACGAAGCGCAGCGTCAGACGCTGGGACCGGTCCGTGGTCACATCGTGGGTCGGGGCCACCGTGGGGTTCCTAAACTTGTTGCCCAGGCTGAAGTACGTGTCGGTGGCGCGGGCAAACTGCACCAGACCCGGGCTCAGGTACTCCGAGGCGTCCTGCCCGGCGATGTGCATGTACGCCCACTGCGGCATCATCGAGGGGGTGGCCATCTTCGCGCGTTCTCTGGCGGTAAGGCACATACAAGTTAAGCTCTCCCTTTAGTGTCCTCTTTCCTCTCTCTCAATAGCATCGGCGGCGCTTCAGACTTTTCACTCCCAGGCCCACGATACTGTGCAGCGTGCTCTGCCAGTTCTGGGCCTGGCGGCGGGCGGGGACGCGGCGCACGGCGGTCGCCACCGGGGGGGCCTGCACAACCGGAGTTGCCTTCGGTGGAGCGGGCGCGGGTGGTGGAGGCAGGTCAAGGGTGGCGGGTTTCATTACACCCGTGGCCAGCGGCGCGATGATGCGCGTGGTGGGCATGCCGGCCTTGACGGCCTCCTCGTACGAGGGCGGCTCGTCCACCTGCAGGATCGTCTCCTCCGCGTCGGGCCGCGGCCGCTTCTCGCCCTTGGGCGGCGGAAGAGTTGCATCTTCCATCTCCACCACCGTGGGCGGTCGCGGGTCCAGGCGGCTGTTTATCTCGCGCTGCACGGCCTGGTTCGCGATGTCCACCACCCCGTTAATGCCCGAGGCCAGCCCGTCCACCACCTTCTGCTGGAAGTTCTGGTCTTTGAGCTTTTCCCTCAACAACTGCCCCGTGCTACTATTCCAGGCCTTGTTCCCATAGGTCCGGAGCGTCGAGCCGAAATTTTTAAGCCCGCTCCAGACACTGCTCCAATTGAAGGCGCCCCCGTTCAGCTGGCTGGTGCCGATCTCGTTCCAGGTGCCCATGAACGGCCGCGTGCCGTGCCGCGGAGCCAGGGACGCAAAATTGATGTCTTCCATTCTAAAAATATACAGGACCAGGTGTGTCAGAGTCTAAAGAGGCTTTATTCGCACATCAACGGCGCTGCGACGCCTGCAGCGCAACGGAAGCTATGCCCGGGATCGTGCCAATGGCCGCGGCGATTATGGGGATGAGCGCGGGCAGAAAGCCGCCACTCAGGCGCCTGCGCATGGCCCGCCGCCGGCGGCGGTTCAGGCCGCTGCCTGCCATGCCTCTCCTGCGGCGGGATCTTCCTCGGTAATTCGGGACGGGGATGCGCAGGCGGCAAGTGAGAGCCATCTCTCTGCAACAGTATCACAGCACACGGATTAAAGGATGCTGGGGTGGTAGCGCACGGTGGGCAGAACGAGCGAGCGGCCCCGGCGCGTGGTCACGCGGCGCACGCGGGCGGGGGCCAGACGGCGGCGGGTGGCGGTGCGGCGGCGGCGTTTGCTGGCTGGGCGCCGGCGGCTGGCGTAGTACCGGGTGCCGCGGTAGCCCGGCGTCGGGATGATGGAAGGATGCAACACGTAGTTGGGCATCAGCCGGCTGGCGGCGCCCCATCTTCGCCTCCTGGGAGGCTCGGTAGCCGTGGCGGCGGTAGAAGTGGAGACGGTAGCGGTGGAGGCTGGGAGCCAGGGGTCGGTTTGCACCTCGATGCTGGTCGAGGGTTTTTTGTCGACATCCATGTCGGTGGGGATCTGAATGTCCACGGTTTGCACGCCCAGGCCCGGCGCCACCTGCTTGATGGGGCGCACCTTGACCTCGGGCTCCACATCCATTTTCACGGTGTCCAGCACGTCCTCCACGCGCCGGCGCTTGGGCACCATGATCTGCATGGTCGGGTACATGCTCTCGCCCTCGCGCTTGACCCCGCGGCTCGGCAGCAGCACCGCCTGGGGCAGCACCTGCTGCAGGGTCACGGGCTTCAGGCTCGGCGTGGGGTTGCTTTCGTCCAGCGGCAGCGAGATCAGCTCCTCTTCGCGCGACCGCTTCCCGTAGGCGAACTCGCCCGCCCGCTCCGCCGCCTGGTCCAGGATGTCGTCGTCGCCGTACACCTCGTCATAGCTACGCTTGCTCCTGACCGAGGACCGCTCGCCGGGCGTGAACACCACCGCCGTGCCGGGCCGCAACACGCGCTGCACCCTGCGCCCCTTCCAATTTACGCGCCGCCGCGGAGCGAACTCGCGCACAAACTCTACTGCCCCCTCGTCCACCTCATCCTTTTTTTTAACCCGCTTGATTTTGCGGGGTTTCTGGTCCGCCTGGGGTGGTCCGTAAATCTCCGGGGCGACGACCTGGAGCATCTCCTCCTTGATTTTGCGCTTTGACATCGTCGCTTGCGGGGGAGGACACAAGCATTAGATCAGGGACGAGGAGGACGGGTGCGCACGGGCACGCGCACGCCCGTGACGGAGTCGCGCACCCAGTACACGTTTCCGCGCCTGGGTCTGGTCATGCTAGAGATGGCCGCAGCGGCGGCGGCGGCCGCTCGTCTGCGAGTCCTGCCTGCGGGGGTGGGTGCAGTGGCAGCGCGGCGCGCGGCTCGCATCATGGCCCGGCGGCCCGTGCGTCTGGCGCGGCGCAGCAGAGCCCGGGCGGCGCGCATGGCGGGCGTGCTCCGGTGGCGCCTGGCGATCCGTCGCCGCCGGCTCTTGCGTCTGGCATAGTCGCGCGCGTCGGCCACCACGCTGTCGATGACCGCGTCCACGGTGGAGGGGGCGGGGGTATAGTTGCGCGCGTCGGCGACCACCGAGTCGATGACGTCGTCGACGGTGGTGCGCACGGCGGCGGTGGAAGTCCGCCCGCGCTTGTATGCGCCCCAGGGAGCGCGGAAGTGGCCGCGGACGCGGACGGGGTGCTGCTGGGAGCGCTTCTTGGCTCCTCCGTACATGGTGCTGGGTCTAGTAAGACCCCAGCCGGTGTTATTGCTGGGCGAGATGAGAATAGACATTTTTTTAGAAGGTGCGACTGGAAAGCACGCGCGGCGCGACTATGCCCAGGGCCTTGTAGACGTAGGGACAGGTGCGGCGACGGGCGTCAGTGACGGTCACTCGCTGGACTCCGCGGATACTGCTGCGCAGCGGTAGCGTCCCGTGATCTGTGAGAGCAGGCACGTTTTCACTGACGGTGGTGATGGTGGGCGCGGGCGGGCGGCAGAGGATCTGGTTGTCGGGGAAGCGGTTGAAGACGTGGGTGAGGGAGGTGTAGCTGCGGATGAGCTGGGAGTAGACGGCGAGGTCGTTGTAAAAGCTCTTGGCGCGGAAGGGCATGAGCTCGGCGCCGACCACGGGGTAGTTGCTGACTTGCTGGGTGGAGCGGAAGGTGACGGGGTCTTGCATGAGGTCCGGCAGTGACCAGTAGACTTGCTCCGCGCCGCAGGTAACGTCCGGGGTGGTGAGCAGCGTCCACGACTGCACCCCCTTCTCGGGGTCCCCGTAGGTATAGGACAGGTACCAACTGCGGTACAGCGTGTCGTGGGTCCCCTGGATCAGGTTGTAACTTCTACCACTCTCATCCTTTTCAATGGGCAAGACTTCTACCTTCTTATCAGCTGCTGTCTCTTGCGCTGGAGTTGCAAATGTATCACCCCTAGTGGTTGTATCGGCTGTGGCCGCAGCTGCATTTTTAGTTTCGTCTTCAACTTTCTTCTTGCTTTCCAAGTACTTGGGCACATCAAGGAGGGCGGGGATGTTGCCACCTTCTAGATCCTCATACATGATTCTGAAGCCCTCTTGGAAAGGTTGCTTCTTGCGAATGCCCAGGAGGTTGCTCAGGCGGCTCTCGGTGAAGTCCACCCCGCAGCCCGGCAGCAGCACCACGTCCGGGTGGAAGGCCTCGTAGGTGTAGACCCCTGGCATCACCAGCTTGGTCACCGGGTCCCAGCCCAGCTTGAAATTTCTGCTGTCAAACTTGACTCCGATATCGCTCTCCAGCACGCCATTCTGCCGCCCCACTTGCAAGTAGTTTTCCAAGATGGCGTTGTTCATCAGGTCTATGGTCATGGTCTCGGAAAAGTTTCCCTCGGGCAGGGTAAACTCAAACCACTCATATTCAAGCTTATCCTGACTCAAATCTGTTTCAACTACCCCCTCTGGATGCTTCCTAGACACCATCACCCGCGCCTTAAACTTGCTGGTGAACATGTACTCGTTCACATTGGGCATGTTAGTGTGCAGAATGGTCTTCAGATCACCGCCCCACCGCGACCGCTCGTCAAAATTTATCGTCTGCGTGCTGGCCTCGGCGGGGGTGAAATCGTTGTTCTGCACCACCGTGGTCAGGAAGTTGCTGTGGTCGTTTTGGTAGTTCAGGGAAGCGATGTCCGCCGACTTGTTGTCCACCAAGTACACGCGAGTGGTGTCGTACTGCGGAGCCAGCTCCGAGTAACGAATGCTGTTTCTGCCCTCCGTAGGAGCCATATACCGCGGAGGCACAAACGGAACCTCCAGGGTCGCCTGCGCCATCACGCTCTCGTACGAGGGAGGAGGAGAGGAAGACACCACCGCGCGCCTCATCTCTAACAAGGAAGAGAACGCACGCTATGGCCTTGGTGAGTTTTATTTCATTCAAAACATCCGTCGCCCCAGTTTGGGCTGCAGATGCGCGAACGGGTTGACCCCACCGCTCCCGCCCAAGTCCAACACGCTGCTATCGTCGGCCGAGTCCTCGTCGTCCCACACCAGGCCCCGCTGCCGGTCGTGGCGCTGTCGCCGCGGCGCGGGCGCGTCCCTATGGTCCTGAGCGTAGGTCTTCCAGCGGTTCAGTTTGTCCACCAGACTCTCTATCCCGTTATTGGCCATGGCGTTCTTGGCCCGCGGCGGCTGCAGCAGCGAGTCGTTCAGGTACTCGTCCTCGCCCAGCAAGCGCGGCCGGCTCACCCTGCCGCTGTTCACCGAGCCCGGCAAGCTATGCAAACTCCCTAAGCTAGGAAAGGGGCTCCTCCCCACCGCGCCCTCGCTCGGCGGCGCCTCCTGGCGCTTTTGAAAGGTCGGCGAGAACACCGCGCTGTCCACGTCGTCCCACAGGAACCCGTCGTTGGGGTCGGGCATGTCGTACTCGCCCGTGTAGAACCCCGGCGGCGGGAGCCAGTGCGGGTTCAGGATGGCGTTGGTAAAGTAGTCCGTGTTCATGGCCGCCGCGCGGTGCAAGTAGTCCATCAGCTTATTGATGAACGGCCGGTTGGCGGCGTACATGCTAGGTTCCATGTTGCGCGCGGTCATGTCCAGCGCGGCGCTGGGGGTGGCACCCTCCTGCATCAGGAACAGCCCTACGCTCTGCTGCACATATCTCAGAATCCTTTCCTCCTCGGCCGACAGTGCGTACTGCGCCGGGATCTTCTGCTGTCTATTGGTCAGCAAAAAGTTCAGGGTGGCCCTCAGACTGTCGGTGTCGTTCTGCCCCAGCGCGCGGCTCACGCTAGTGATCTCCTGGAAGGTCTGCTCGTCCACCTGCGCCTGGCCTATGGCCTCGCGGTACAGCGTCAGCAGATGGCCCAGGTACGAGTTGCGGTTCACGCTGCCGCTGTCGGTGAAGGGCGCGATCAGCAGCAGCAGCAGCCGCGAGTTGGGCGTCAGCAAGCTGCTCACCGTTGACCGGTCGCCCACGGGCGCCTGCACGCCCCACAGCCCGCGCAGATTCTTGAAAGCCTGGCTCAGGTTCACCGTCTGCAAGCCCTGCCGGCTCGTCTGGAAAAAGTAGTCCGGGCCAGACTGGTACACCTCGCTCTGGGGCACCTCGGTCACCATCAGCCGCAGCGCGCTGATAAAGTTGGTGTAATCGTCCTGCCCGCGCGGCACGTTCGCCGGCTGCGTCGCCAGGAAGGCGTTCAGCGCCACCAGCGAGCCCAGGCCCTCGTTCTTGAACCGCTCGCGCTGCGCCACGGCCTCGCGCACGTCCGTCACCAGCCGATCCAGGTTGGACTGCACGTTCGTGCTGTTGTAGCGGCCCACGCGCTCCAGCAGGGCGTTGTACACCAGCCCGGCCTCGTCGGGACGGATGGCCTTGTTCTCCGCCAGCGCGTTCACGATCGCCAGCACCTTCTCGTGCGTGGGGTTGGTCCGAGAGGGGACCACCGCCTCCAGAATGGCCGAGAGTCTGTTGGCCTGCGGCTGTTGTCTAAAGGACTCGGGGTTGCGGGTCGTCAGGGCCATGATGCGTTGCATCGCGGCCTCCCAGTCGTCCGATGCTAGACCGGACGGCTGGCTTTGCAGCGCCGCCCTTATGGCGGGGTCCGGGGCTTGCTGGGACATCTAAAAACACATCACGGAGGCGTCAGTACCCCGCAGCGGGTGCATCCTCCTCCTCTTCCTCTTCCTCATCCAAGTCCTCTGAACCGTAGGCGGCCTCCAAGGCGCGCCGGCTCGGCTGCCAATGCAGGTCCGCACCCATGTCGAAGTAGGACTCCTCGCCGCCGGCGGCGCCCCCTACCAGCGCCCGGCGCAGACTCAGCATCAGCTCGCGGTCGCTCAGCTCGCGCCGCCGGCTCGTGCTCACGGCCTTGTGGATGCGGTCGTTGCGGTACACGCCCAGGTCGTCGCTCAGCGTCAGCACCTTGAGCGCCATGCGCATGTAAAAGCTGTCTATCTTCACCTCCTTGTCTATGGGCACGTACGGCGTCTTGTAAATCTTGCGCGCGTAAAACTTGCCCAGGCTCAGCACCGAGTAGTTGATCGCCGCCACCTTCTCGGCCAGGCTCAGGCTGCGCTCCTGCACTACGATGCTCTGCAAGATGTTAATCAGCTCCAGCAGCCAGCGACCCTCGGGCTCGGCGATGTTCAGCAGCGCCTCCCTGAACGCCTCGTTGTCCCTGCTGTGCTGCACCACCAGGAACAGCTGCGCCGTCAGAGGCTTGCTGTCCGGGTTCTGCACGATGGCCTCCGCCAGGTCCCACAGGTGCATCAGGCCCAGGGCCACCTCCTCGCGCGCGATCAGGGTGCGCACGTGGTTGTTGAAACTCTTTTGGAAGTTGCGCTCCTGCTTCACCGTCTGCTCGTAGGCCGTCACCAGGTTGGCTGCCGCCACGTGCGCGCGTGCGGGGCTGATCCCCGTCTGCTCGTTCGGCTCGAAATCCTCGTCGCGCAGCACGCGCTGGCGGTCCAGGCCCTCGCGCAGCTCCCTGCCCGCCCGAAACCGGCAGTCGCGCATCTCCTCGGGCTCCTCCCCGCTGCGGTCCCTGAACAGGTTCTGCGTAGGCACGTACGCCGGGCGCACGTCCTTCTGCAGCTGCACGCGGGGATGTCGCTCCGGGGACGGCGCCCCCAGTCTTGCCAGCCCTTCGCCCTCTTCCAAGTCCATCTCTGTCTGTGGTGGCTGGCTAGCGCCGGCGCCTGCTACGGCCGCGGTCGCGGTGGTCGCCGGGGGGGTGGGACGCATTTGGCGCAGGACGGGATGCATCTGGCAAAAAGAAAAAAGGGGGCTCGCTCCCGTAACTGGAGAAGTCGGCTGGCTGTGGGTCTTTATATCGGCGGGGTGACCAAGTCCCGCTCGCCGCGGCCGTCCTCGAACCGGGTTCTGCCGCGACTCAACCCTGGCGATGCTTCTCCAGACTACGGGCGCGAGCCACTACCCGGCAGGGTTTTCGGTCGCTTTCAAGTCTAGCGACCCCACTCGGCCGGCAAAAAGGGCTCTCCGCCGTATCCTGGCGGCTATCGGGCTCGGGTCGAGACGGGAGTGCCAATACCACGTTAGTCGCGGCTCCAGCCTGATTCGAGGGGACTCGAACCGGGGTACACACGCGGCCTAACCCGTTTGCGTTCCGCCAGGCTACGGAGGAAGAGCCCGCTCAACCGCTTTCGTTTTTGCCTCTAGAGCGTCAATGACTGCGCGCGTCTCACCGGCCAGACCGTGCCGACCATGGACTATTTCCTGCCGCTGCGCAACATCTGGAACCGCGTCCGCGAGTTCCCGCGCGCCTCCACCACCGCCGCCGGCATCACCTGGATGTCCCGCTACCTCTACGGCTACCACCGCCTCATGCTCGAGGACCTGGCCCCGGGCGCGCCGGCCACCCAGCGCTGGCCGCTCTACCGCCAGCCGCCGCCGCACTTCCTAGTCGGATACCAGTACCTCGTGCGCACCTGCAACGACTACGTCTTCGACTCGCGCGCCTTCTCGCGGCTCAGGTACTCCGAGGTCGTGCAGCCAGGCCTGCAGACCGTCAACTGGTCGCTCATGGCCAACTGCACCTACACCATCAACACCGGGGCCTATCACCGCTTCGTCGACATGGACGACTTCCAGGACACCCTCACCCGCGTGCAACAGGCCATCCTCGCCGAGCGCGTCGTCGCCGACCTGGCGCTCGTGCAGCCGCTCAGGGGCGTCGGGGTCACCCGCATGGAAGACTCCGCCTCAGCCAGTGATGACATTGAGAGGCTCATGCATGACTACTACAAGAACCTGAGCCGGTGTCAGGGCCAGGCCTGGGGCATGGCCGAGCGGCTCCGCATCCAGCAAGCTGGACCCAAGGACCTGGTCCTCCTCGCCACCATCCGCCGCCTCAAAAACGCCTACTTCAATTACATCATCAGCAACCGCAATTCTAACAGCGTCCGCAGGGCTGCTACGTGTTTGAGCTTACCTTGCGACTGCGATTGGCTAGACGCTTTCCTCGAAAGATTCTCCGATCCGGTCGATCTCGACGCGCTCACGTCCCCTACACCGCAATTGATAAGATGCATCGTCAGCGCCCTATCGCTGCCCAATGGGGACCCGCCCCATTACCGGGAGATGACCGGCGGCGTCTTCACGCTGCGTCCTCGCGAACGGGGTCGCGCCGTCACCGAAACCATGCGTCGCCGCCGCGGGGAGATGATCGAGCGCTTCGTCGACCGTCTCCCGGTGCGTCGCCGTCGTCGTCGGCCCCCGCCACCACCACCGCCCCCAGAGGAAGAAATAGAAGAAGAGGTCGTCATGGAAGAAGAGGAAGAGGAGGAGGCCCCCGGGGATTTCGAGCGCGAGGTGCGCGCCACCATCGCCGAACTCATCCGGCTCCTGGAAGACGAGCTCACGGTCTCGGCCCGCAACGCCCAGTTTTTCAACTTCGCCGTGGACTTCTACGAGGCCATGGAAAGGCTGGAAGCCATCGGCGACATCAGCGAGATGCCCCTGCGCCGCTGGATCATGTACTTCTTCGTCACCGAGCACATCGCCACCACCCTCAACTACCTCTTCCAGCGCCTGCGCAACTACGCCGTCTTTGCGCGGCACGTGGAGCTCAACCTCGCGCAGGTGGTCATGCGCGCGCGCGACGCCGACGGGGACGTGGTCTACAGCCGCGTCTGGAACGAGAGCGGCCTGGGCGCCTTCTCGCAGCTCATGGGTCGCATCTCCAATGACCTCGCCGCCACCGTGGAGCGCGCGGGCCGCGGCGATCTCCAGGAGGAGGAGATCGAACAGTTCATGTCCGAGATCGCCTACCAGGACAACTCGGGCGACGTGCAAGAGATCCTGCGTCAGGCCGCCGTCAATGACGCAGAGATTGATTCTGTTGAACTGTCTTTCAGGTTCAAAGTCACGGGGCCCGTGGTCTTCACGCAGAGGCGGCAGATCCAGGATGTCAACCGCCGCGTCGTCGCGCACGCCAGCGCTCTCAGGGCGCAGCACCGGGACCTGCCCGAGCGCCACGCCGACGTGCCTCTGCCGCCCCTGCCCGCGGGGCCGGAACCGCCGCTGCCGCCGGGAGCGCGTCCGCGACACCGCTTCTAAAAGCGCACCGCGGCACGGTCGTGGCCCCGCGCAGCTACGGGCTCATGCAATGCGTGGACACGACCACCAACTCACCCGTAGAAATCAAGTACCATCTGCATCTCAAGCACGCCCTCACCCGCCTCTACGAGGTCAACCTCAGAACCCTGCCCCCGGACCTGGATCTCCGCGACACCATGGACAGCTCCCAACTGCGCGCCCTCGTCTTCGCTCTCCGCCCCCGCCGCGCCGAGATCTGGACCTGGCTCCCGCGCGGGCTCGTCAGCCTCTCCGTCCTCGAGGAGCCCCAGGGTGAGTCCCACGCAGGCGAACATGAAAACCACCAGCCAGGGCCGCCACTCCTGAAGTTCCTCCTCAAGGGACGCGCTGTGTATCTCGTGGATGAGGTACAGCCCGTGCAGCGCTGCGAGTACTGCGGACGCTTTTACAAGCATCAGCACGAGTGCTCGGTTCGCCGGCGGGATTTCTACTTTCATCACATCAACAGCCACTCGTCCAACTGGTGGCAGGAAATCCAGTTCTTCCCAATCGGCTCTCATCCTCGCACGGAGCGGCTCTTTGTCACCTACGATGTAGAAACCTACACCTGGATGGGGTCCTTCGGCAAGCAGCTCGTCCCCTTCATGCTGGTCATGAAATTCTCCGGGGACCCAGAGCTGGTCGCCCTCGCTCGCGATCTCGCCGTGCGTTTACGCTGGGATCGCTGGGAGCGGGACCCCCTCACCTTCTACTGCGTCACCCCAGAAAAGATGGCCGTGGGCCAGCAGTTCCGCCTCTTTCGCGACGAGCTCCAGACCCTCATGGCCCGCGAGCTCTGGGCTTCCTTCATGCAAGCCAACCCACATCTCCAGGAGTGGGCGCTCGAGCAGCACGGCCTGCAATGCCCCGAGGACCTCACCTACGAGGAGCTCAAAAAGCTGCCGCACATCAAAGGCCGCCCGCGATTCATGGAACTCTACATCGTCGGGCACAACATCAACGGCTTTGACGAGATCGTCCTCGCCGCCCAGGTCATCAACAACCGAGCCTCCGTCCCGGGCCCTTTCCGCATCACCCGCAATTTCATGCCGCGGGCAGGCAAGATTCTCTTCAATGACGTCACTTTCGCTCTGCCTAACCCCCTCTCGAAGAAGCGCACCGATTTCGAGCTCTGGGAGCACGGCGGCTGCGACGACTCGGACTTCAAGTACCAGTTCTTGAAAGTCATGGTCAGGGACACCTTTGCCCTGACGCACACCTCGCTCCGCAAGGCCGCTCAAGCTTACGCCCTCCCCGTGGAGAAGGGCTGCTGTCCCTACAAGGCCGTGAACCATTTCTACATGCTGGGCTCTTACCGTGCGGACGATCGAGGATTCCCGCTCCGGGAGTACTGGAAGGATGACGAGGAATACGCCCTCAACCGCGAGCTGTGGGAGAAGAAAGGAGAGGCGAGTTATGACATCATCCGCGAAACGCTGGACTACTGCGCCATGGACGTCCTCGTCACCGCCGAGCTCGTCGCCAAGCTGCAAGACTCCTACGCGCACTTCATCCGCGACTCGGTCCGCCTGCCTCACGCCCACTTTAACATCTTCCAACGGCCCACCATCTCCTCCAACTCGCACGCCATCTTTCGCCAGATCGTCTTCCGCGCCGAGCAGCCCCAGCGCACCAATCTCGGCCCCGCCTTCTTGGCCCCCTCGCACGAGTTGTATGACTACGTGCGCGCAAGCATCCGCGGGGGGCGCTGCTATCCCACCTACATCGGCATCCTCTCGGAGCCCATCTATGTCTATGACATCTGCGGCATGTACGCCTCCGCCCTCACGCATCCCATGCCTTGGGGTCCGCCCCTCAACCCCTACGAGCGAGCGCTGGCCGCCCGCGAGTGGCAGATGGCCTTGGACGATGCATCCTCAAAAATCGATTATTTTGACAAGGAACTCTGTCCGGGCATCTTCACCATCGATGCGGACCCCCCTGACGAGCATCTGCTTGATGTGCTGCCCCCGTTCTGCTCGCGCAAGGGCGGCAGACTCTGCTGGACCAACGAGCCCCTGCGCGGCGAGGTGGCCACCAGCGTGGACCTGGTCACCCTGCATAACCGCGGCTGGCGCGTCAGGATCGTGCCCGACGAGCGCACCACCGTCTTCCCCGAATGGAAGTGTGTCGCGCGCGAATATGTCCAGCTCAACATCGCGGCCAAGGAGCGCGCCGACCGTGACAAAAATCAGACCATGAGATCCATCGCCAAGCTTCTCTCAAACGCCCTCTATGGCTCCTTCGCCACCAAGCTTGACAATAAAAAAATAGTCTTTTCTGACCAGATGGATGAAAGTCTCCTAAAAAGCATCGCGGCAGGGCAGGCCCACATCAAATCCTCCTCGTTTCTAGAAACTGACAACCTGAGTGCCGAGGTCATGCCCGCTCTTGAGAGGGAATACCTACCCCAACAGCTGGCGCTCGTGGACAGCGACGCGGAAGAGAGTGAGGACGAGCACAGACCCGCCCCCTTTTATACCCCCCCGTCGGGAACCCCAGGTCACGTGGCCTACACCTACAAGCCAATCACCTTCTTGGATGCGGAGGAGGGGGACATGTGCCTGCATACCGTGGAAAAGGTGGACCCCCTGGTGGACAACGACCGCTACCCCTCGCACGTGGCCTCCTTTGTCTTGGCGTGGACGCGCGCCTTCGTCTCAGAGTGGTCTGAGTTTCTCTACGAGGAGGACCGCGGGACGCCCCTGCAGGACAGGCCCATCAAGTCCGTCTACGGGGACACAGACAGCCTGTTTGTCACCGAGCGCGGACACAGACTCATGGAGACGCGAGGTAAGAAGCGCATCAAAAAGAACGGGGGAAAACTGGTTTTTGACCCCGAGCAGCCCGAGCTCACCTGGCTAGTCGAGTGCGAGACCGTCTGCGCCCACTGCGGAGCGGACGCCTTCGCCCCCGAGTCCGTCTTTCTCGCGCCCAAGCTCTACGCCCTGCAATCCCTCCTCTGTCCCGCCTGCGGGCGCTCTTCCAAGGGCAAGCTCCGCGCCAAGGGCCACGCCGCCGAGGCCCTCAACTACGAGCTCATGGTCAACTGCTATCTCGCCGACGCGCAGGGCGAAGACCGTGCCCGTTTCAGCACCAGCAGGATGAGTCTCAAGCGCACCCTTGCAAGCGCCCAGCCCGCGGCCCACCCCTTCACCGTGACGGAGACCACCCTCACGCGGACCCTGAGACCCTGGAAGGACATGACGCTGGCCGCGCTGGACGCCCATCGTCTCGTGCCCTACAGTCGCAGTCGTCCCAACCCCCGAAACGAGGAAGTCTGCTGGATCGAGATGCCGTAGAGCACGTCACCGAGCTCTGGGACCGCCTGGAGCTCCTCTCGCAGACCCTCGCCAAGATGCCCATGGCCGACGGGCTCAAGCCCCTGAAAAACTTTGCTTCCCTGCAAGAGCTTCTCTCGCTGGGCGGGGACCGCCTCCTCGGCGAGCTCGTCCGGGAAAACCTCCAAGTCAGAGACAAGCTCAACGAGGTGGCCCCCCTCCTCCGGGACGACGGCAGCTGCATGTCCTTGAACTACCACCTGCAACCCGTCATCGGGGTCATCTACGGCCCGACCGGGTGCGGCAAGTCCCAGCTGTTGAGAAACCTGCTCTCCTCGCAGCTCATCACCCCCGCCCCGGAAACCGTTTTTTTCATCGCCCCGCAGGTGGACATGATCCCCCCCTCCGAGATGAAAGCCTGGGAGATGCAGATCTGCGAGGGGAACTTCGCCCCGGGGCCCGAGGGAACCATCGTCCCCCAATCTGGCACCCTCCGCCCCAAATTCATTAAAATGTCTTATGATGATCTCACCCAGGAGCATAATTACGATGTCTCTGACCCCAGAAACGTCTTTGCCAAAGCCGCAGCCCACGGGCCCATTGCCATCATCATGGATGAGTGCATGGAAAACCTGGGCGGGCACAAGGGCGTCTCCAAATTCTTCCACGCATTCCCTTCCAAGTTGCATGACAAGTTCCCCAAGTGCACGGGCTACACCGTTCTGGTGGTCCTGCACAACATGAACCCCCGGCGGGATCTGGGTGGCAACATCGCCAACCTCAAGATCCAGGCCAAACTGCACATCATCTCCCCCCGCATGCATCCCTCCCAGCTCAACCGTTTTGCCAACACCTACACCAAGGGGCTCCCCGTGGCCATCAGTCTCCTCCTCAAGGACATCATCCAGCACCACGCCCAGCGCCCCTGCTATGACTGGATCATCTACAACACGACCCCAGAGCACGAGGCCATGCAGTGGTGCTACCTCCACCCACGGGACGGGCTCATGCCCATGTACCTCAACATCCAATCCCACCTCTACCGGGTCCTGGAAAAAATCCACCGCACTCTCAATGATCGGGAGAGGTGGACCAGGGCCTACCGCGCGCGAAAAAATAAATAATAAAGATGCTTTGCTGTTTGAATCAGAATCTGTGTTTATTGAATCATTTATTTTGCTGCTGCTGCTGTTCGCGGAGCTCGGACACCTGCTGGGTCAGGGCGGCCAGCTGGCGGCTCAGGGCTTCCAGCTCGGCCAGCAGGGCCAGCAGTTTCTCCTCGGCGATGATGGAACTGGGCACAGAGGGGCTACTACCGCTGCTGGGCATGTAGCTCGAGGCCAGTCTCGTCGCTGTCATGGCGGCTGCGGCTGCCGCGGCTGCGGCGGTGCTGTCGAGCGACGAGTTCCCCACGGTCGCGTAGGTCATGGTCGAGGAATTTGCTGGAAGCACTGGGCGCCCATCCACCGTCGATCCCATCACATTCTGACGAACTCCGGCCCATCCCGGCAGGCGGGTTGTCAAATAAGGGCTAAAAAGCCCCCCTTCGAAGGCCCCGCCAGTCCCGTTCATGATGTCTGCAGAAAAGCAAAAAGACCCTCGTAAGACACCCGCCTTTATAGTCACCTTAGCCACGCCCACTACTCAAACCTACCTGCGATCGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTCGGATCTCGTCTAGATTATCATGTGTAGTGCAGTTTCACGCCCTTCAGCACGGGCTCAGAATCGTCCTCGTCGAACTTGCAGCAGCTGCCACAGCTACAACAGCCCTTCAGGCAGCTACAGCAGCTGGTCATGCAACACAGCATGATTGTGACCATCACGATGGCAATCAGTCCGGCGATAAAGCCCAGCCAGATGTACCAAGGCCACTTGATGTACTGCTCGTATTTTCCCAGTTCTTGCAGGTCGATCAGGCTCTCGTTCAGATTCTTGGCCACCTCGTTCAGCCGGTCGATCTCTTTCTGGATGTTCACGACGCTGGCATTGATTCCGCTGATATCGCCCAGGTCCACGTCGGGGCTTGTGTGGTTCTTAAAGTACTTGTCCAGTTCCTCTTTGAAGCTGTCCAGCTCGGGCTGCAGAGGGTCGTACACGGTATTGTTCACAATGCCGATCACGACGTCGCAGTTGCCAGACACGAAGGTGTTGTCGGTGGTGATGATCTGGGGCTCGTAGAAGTTCCGCTGTGTCACGAACCAATGGGTGCCGTTGGACACGAACACGCCTTCTCTAGGAAAGTGGGCTTTGCCGTCGTGGCAGATGGCTGGAGCGGTGGTGAAATTCTTCTCTTGAGCGGGCACATATGTCACGTGCAGAAACACCACGCCGTGAGGGGCAGACTGAGGGAAGCTCATCAGGTGGTAGCCCTTGCCGCAAAAGTCCACTCTCTTGCTCTGGCCCAGCACACACTCAGACATCTTGGTGGCGGCCAGATTGGCAGAGGCTCTAATCTCGGCGGCTCTGATCAGCTGCTGGGTAACGTAGGTCTGCAGGGACTGCAGCCTTCCGGTGATCAGTCTGTCGATCTGCACCTCGGCCTCAGGAGGGTCCAGTCTGCTCAGGATATCGTTCAGCACAGAGCTGATGGCGCCGAAGTTGGAGGACAGCTGCTTGACCAGGGTGTTCAGTGCCTGGGCATTCTGGTTGACCACGTCCTGCAGCTTTCCCAGGGCGCTTGCTGTGCTGCTCAGGCTGTCCTGGATCTTGCCGATGGCGCTGTTGAACTGGTTGGCGATCAGCTTCTGGTTCTCGTACAGCACATTCTGGGTCACTCCGATGCCGTTGAACCGGTAGGCCATCTGCATAGCAAAGGGGATCTGCAGAGCGGCGCCAGCTCCAAATGTCCAGCCGCTTGTGATTGTGCCGGCCAGCAGGGCAGATGTGTACTGGGCGATCATCTCATCGGTCAGCAGAGGAGGCAGCACTGTCAGTCCGTTAAACTTCTGGGCGCAAATCAGATCCCTGGCGGCAATGTCGCCCAGACAATCGCCATACTGCTTGATGAAGCCGGCGTCGGCCAGTGTCACTTTGTTGAACAGCAGGTCCTCGATGAAGCTCCGCTTGCTGGGCTTGCTAGGATCGGGCAGAATCTGGCTGAAATTGAAGCCGCCGAAGTCCTTGATAGGAGGGGTCTTGTAGATCTGCTTCACTTGGGCGAACACCTCTTGGGTGTTCTTGTCCTGTTCCACGGCGATCCCTGTCAGGGCTCTATTCAGCTGGGTGCAGAAGCTGCCGTACTGCAGCAGCAGGTTGGAGCACTCGGTGGAATCGCCGCAGATGTACATGGTGCAGTCCACGCTGGTCTTGGTCATGGACACAGGCAGGATCTCTGTGGTCACGCTGATGGTGAAGTTGGTGGGGATAGCGATAGAGTTGTTGGAGTAGGCCACGCTGTTCTCGGCGCCCAGAGACATTGTGTAGGCAATGATGCTCTGGCTGGCCACAGATCCGGCTCTGCTGGGGCTGTTTGTCTGTGTCTGGTAGCTGGCACAGATGCCAGCGCCGATGGGGATGTCGCACTCGTAGCTATTGTTCACGTGCTCGGCTCCGATCAGACAGCCGGCTCTGGTCTGAAACACATTGCTGCCGGTGGAGTACACCCGCCATGTAGGTGTCAGCTGATCGGCGTGAATGGCCACGGGCACTTCGGTACAGTTCACGTCCTGGTACAGCACTGCCACCTGATTGCTGGTGTTGGTGCCAGGGGTGATCACAGACACTCCGCCGAAGCTGCAAGGGGTGATGTCCAGGATTTCCAGTGTCTGGGGATCTCTAACGGCGTCTGTGGTATCGGCGATATCCCGGCCAAACTGCTGGAATGGCAGGAACTTCTTGTTGCTCTCTGTCAGCACGCCGGTGCCGGTCAGGCCGTTGAAGTTGAAGTTCACGCATTTGTTCTTCACGAGATTGGTGCTTTTCTTAGGGCCGCACACTGTGGCAGGGGCATGCAGCAGTTCGAAGCTCAGCACCACCACTCTGTAGGGCTGATAGCCCACGCCATTTGTGGGCTGAAAGCCGTAGGACTGCAGTGGGAAGTAGCAGTTGAAGCCTTCCACGCCGTTACAAGGGGTGCTGCCGGCCTGATAGATCTCGGTGGAGATGTCCCGCTCGAAGGGCTTCAGATTGGACTTCCGGAACAGCCGGTACAGGTAATTGTAGTTGCCGCCGACTTTGGAGTCCAGGTTGTTGCTGTTCCAGGCAATCACACAGCCGGTGAAGTCGTCGGGCAGCTTGTAGTTGTAGTCGGCGATCTTGCCAGTCTGTCCAGGGGCAATCTGCCGCACTTCATCTCCCCGGATCACGAAGCTGTCGGCGTACACGTTTGTGAAGCACAGGTCGTTCAGCTTGGTAGGGGACACGCCGTAGCACTTGAAGGTGCTGAAGCTGGCGGAGTTGTACAGCACGGAGTAGTCGGCCACGCAATTGCTGATCCGCTTCCGGTTCCAGGCGTACACAGAGGCGAATCTGGTGGCATTGAACACCTCGCCGAAGGGGCACAGATTGGTGATATTGGGGAACCGCACGATGGATTCGGTGGGCTGCACCCGGAAGTTGCTGGTCTGGTAGATGCCCTTTTCCACGGTGAAGGACTTCAGGGTGCACTTTGTCTCGCTCAGAGGATCCAGAGCACAATCCACGGCGTCGGTGATGGTGCCGTTCTCGTTGTACTTCAGCAGAAAGGTTCTAGGCTGCAGGTAGCCCACATAGTAAGCGGCGGCACCAGCTGTCCATCCGCTGCTGCTATCGCCAGGTGTCAGGTAGCTTCTGTGCAGGGCCAGCAGTGTCTGAAACCGGGTGATGTTGATGCCGATGGGCAGATCCACCAGGGGTTCCAGAGCAGAGAAGCCCTGAGGCAGATCCCGCACGAGGTTGATAGGGGTGTGCTTGCTGTAGATCTTGAAGTAGCCGTCGATGTTCTTGAACACGAACTCGCGCAGGTTCTTGAAGTTGCCCTGCTTGCCTTCCAGGTCCATCAGGAAAGGCTGGGACACGTATTCAAAGGTGCAGTTGTTGGCGCTGCTGTACACCCGGAACTCGCTTTCCATCCAGCTCTTGTTGTTCTTGTGATAGTAGACGCCCAGGAAGGGGTCGTTGCAGAACTGGAACTCGCACACTTTGATGACCACGTTGGTGGCGTTGTTCACGATCAGCAGGCTCTGGGTCTTGCTGTCCAGTGTGGTGCCGAAGATCCAGCCTCTGATGATGTTGGACTTCTCGGTGCTGGCAAAGTACACCCCGTCGTTGAAGGGCAGCACGGGGTTGTCGAATCTCTTGGTGCCATTGGTGCCGGACACGTGGATGGCGTGGAACCAGGTCACGTTGCTGAAGAAAGGCAGGAACAGGTCCTGGGTAGAGTGCAGCACGCTGGATCTGAACACCTTGTCGGGGTAGTACACGCCTCTGGTAAAGCTGTTGGTGTAGGCTGGAGGCAGCTGGGTTCTTGTGGTCAGGTTCACGCATTGACTGGAGACGAGGGGGAGCAGTACCAGAAACACGAACATGGTGGCAAGCTTCCAATGCACCGTTCCCGGCCGCGGAGGCTGGATCGGTCCCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGGCGTCTCCAGGCGATCTGACGGTTCACTAAACGAGCTCGTCGACGATCTCTATCACTGATAGGGAGATCTCTATCACTGATAGGGAGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGCGGAACTCCATATATGGGCTATGAACTAATGACCCCGTAATTGATTACTATTAATAACTAGTCAATAATCAATGTCAACGCGATCGCTCAGCTGATCTGTGACATAAACACCGGACTTTGACACGGACGCGGAAATTCGCGAAAAAAACACCGCAATCGAAACCCCCACGTAATCGGTCAAAGTCTCTCGGCCCTCGGTAAATATTCCGCCCTGGGAGGGACCGGTATTTTTCGCTTTTCACTTCCTCATTCAGTTTTCGCGCCAAAATGACCTAATTTCACTTGCATCCGCCCAGAATTACCTCATATCCGGGCGTGGCCGCGGGAAAATCGGCCGTTTCCGGGTCTAAAGTCCGCTTTTTTATACGTCATCAGCCCCGCGACTTGCTTCCGGGCTAGGCCACGCCTCCGCGGCGACCGTTAGCCGTGCGTCGTGACGTCATTTGCATCACCGTTTCTCGTCCAATCAGCGTTGGCTCCGCCCCAAAACCGTTAAAATTCAAAAGCTCATTTGCATATTAACTTTTGTTTACTTTGTG

BLAST Hit for Human Adenovirus 26

Figure 2. BLAST of the 33kb contig against NCBI returns a 23,322bp perfect match to Human adenovirus 26.

Figure 3. Pfizer vaccine sequence aligned to Ad26.COV2.S (J&J). Hash marks on the top arrow depict variants in their respective codon optimizations.

Figure 4. IGV view of over 9500X coverage across 33kb. Red tick marks are read pairs with larger than average inserts. Unless they line up vertically, they are just insert size outliers in the library and not reflective of an insertion or deletion.

Methods

Sequencing was performed as described previously. Briefly, DNA was isolated using a CTAB/Chloroform/SPRI method. Sequence libraries were constructed with Watchmaker Genomics Illumina Fragment library kit. Libraries were sequenced on an Illumina HiSeq4000. Reads were trimmed with TrimGalore and assembled with MegaHit. The largest contig was annotated with SnapGene.

Discussion

The Spike sequence in the J&J Ad26.COVS.2 vaccine has a different codon optimization than Pfizer BNT162b2. This can impact Primer design for qPCR assays attempting to Target multiple vaccines. MGC Spike qPCR and RT-qPCR primers described previously are a 100% match. The MGC Ori Primers only target the Bacterial Origin in Moderna and Pfizer monovalent and bivalent mRNA vaccines. The Ori qPCR/RT-qPCR assay does not hit this Adenovirus vector.

Sequence Data

BAM File

BAM Index File

Fasta_File

SnapGene File

*TheNZMH destroyed my MEGA account for mirroring Steve Kirsch’s data. We are working to restore these link but in the meantime, we are working with an IPFS interface.

https://coffee-urban-barracuda-866.mypinata.cloud/ipfs/QmSyv2iJscNuVCuXbpyxu2yMJ2YdeqpV1ZLke8h5L7B5BX/?pinataGatewayToken=IHKPPgHulHekwLshClUsau25l5NAoVG58EuJbKX1SIBboZjyvk5ZKksVzlp7Td1Z&_gl=1*1ul9sd6*_ga*MTA0MDQzODk2MS4xNzAxNzQ4OTg0*_ga_5RMPXG14TE*MTcwMTc0ODk4My4xLjEuMTcwMTc1MDA2MC42MC4wLjA.

Acknowledgements